%PDF-1.3
%
3 0 obj
<>>>/BBox[0 0 612 792]/Length 133>>stream
xͱ EᝧRhWG4A)C!ooYpX3H\&& q8K݅jfIv?ttxD"(pבּ1-ϾtȞcƕȫqUT)x
endstream
endobj
5 0 obj
<>>>/BBox[0 0 612 792]/Length 133>>stream
xͱ EᝧRhWG4A)C!ooYpX3H\&& q8K݅jfIv?ttxD"(pבּ1-ϾtȞcƕȫqUT)x
endstream
endobj
4 0 obj
<>>>/BBox[0 0 612 792]/Length 133>>stream
xͱ EᝧRhWG4A)C!ooYpX3H\&& q8K݅jfIv?ttxD"(pבּ1-ϾtȞcƕȫqUT)x
endstream
endobj
1 0 obj
<>>>/BBox[0 0 612 792]/Length 133>>stream
xͱ EᝧRhWG4A)C!ooYpX3H\&& q8K݅jfIv?ttxD"(pבּ1-ϾtȞcƕȫqUT)x
endstream
endobj
7 0 obj
<>stream
application/pdf
MAB-2018-0006-ver9-Yamada_3P 162..165
2018-06-23T07:09:27+05:30
Arbortext Advanced Print Publisher 9.0.114/W
2018-06-29T07:15:20-07:00
Acrobat Distiller 8.1.0 (Windows); modified using iText 4.2.0 by 1T3XT
uuid:e61f60e0-26dc-4d9d-b6e9-05688ec6bdc6
uuid:e7c23637-1243-443c-89aa-0424feef527d
endstream
endobj
8 0 obj
<>stream
x+ |
endstream
endobj
9 0 obj
<>stream
xS**T0T0 Bi yA$
endstream
endobj
10 0 obj
<>stream
x+ |
endstream
endobj
11 0 obj
<>stream
xS**T0T0 Bi y8#
endstream
endobj
12 0 obj
<>stream
x+ |
endstream
endobj
13 0 obj
<>stream
xS**T0T0 Bi yS&
endstream
endobj
14 0 obj
<>stream
x+ |
endstream
endobj
15 0 obj
<>stream
xS**T0T0 Bi yJ%
endstream
endobj
17 0 obj
<>stream
/GS1 gs
BT
/F3 1 Tf
9.843 0 0 9.843 59.7543 725.1588 Tm
0 0 0 rg
-.0205 Tc
0 Tw
[(C)-5.3(l)1.3(ara,)-191(C)-5.3(A).6(\).)-196.7(The)-195.5(e).3(nzyma)-5.5(tic)-195.5(react)]TJ
12.2855 0 TD
-.0198 Tc
[(ion)-187.9(w)-4.5(as)-193.5(conduc)-4.8(ted)-193.7(u)2.1(sing)-187.9(1-Step)]TJ
-12.2855 -1.1174 TD
.0111 Tc
[(Ul)4.1(tra)-210(T)-2.7(MB-)4.3(E)-2.7(LIS)4.4(A)-215.5(\()4.3(T)-2.7(hermo)-214.6(F)4.4(is)4.4(her)-214.6(S)-1.4(cient)4.1(i)-1.6(c,)-211.2(I)-1.5(n)4.2(c)-2.7(.\).)-211.2(O)3.4(pt)4.1(ical)]TJ
0 -1.1116 TD
.0131 Tc
[(de)5.1(nsi)6.1(t).4(y)-247.2(w)5.4(as)-247(m)5(e)-.7(a)5.1(s).7(u)6.2(r).5(e)5.1(d)-247.2(at)-247.3(6)6.2(5).5(5)-160.8(n).5(m)-248.4(u)6.2(si)6.1(ng)-247.2(a)5.1(n)-247.2(iM)5.3(ar)6.3(k)-247.2(m)-.7(i)6.1(c)-.7(r)6.3(o).5(p)6.2(l).4(at)6.1(e)]TJ
T*
.0112 Tc
[(reader)-370(\()4.4(B)-2.4(io)4.3(-Rad)-364.3(Labo)4.3(rat)4.2(o)-1.4(r)4.4(i)-1.5(es,)-366.6(I)-1.4(n)4.3(c)-2.6(.,)-366.6(Berkeley,)-366.6(CA\).)-366.6(These)]TJ
T*
.0096 Tc
[(re)-4.2(ac)-4.2(tions)-152.6(w)-3.9(ere)-153.9(p)-3(erform)-4.2(ed)-158.5(at)-152.9(37)]TJ
/F9 1 Tf
12.3546 0 TD
0 Tc
()Tj
/F3 1 Tf
.4089 0 TD
.0117 Tc
[(C)-151.7(w)-1.8(i)4.7(t)-1(h)-150.7(a)-151.8(to)4.8(tal)-156.6(s)5(ampl)4.7(e)-151.8(vol)4.7(ume)]TJ
-12.7635 -1.1174 TD
.0126 Tc
[(of)-247.7(50)5.7(1)5.7(00)]TJ
/F8 1 Tf
4.3313 0 TD
0 Tc
(m)Tj
/F3 1 Tf
.5645 0 TD
.008 Tc
(L.)Tj
/F7 1 Tf
8.9663 0 0 8.9663 59.7543 642.9542 Tm
0 Tc
[(Flow)-338.6(cytometry)]TJ
/F3 1 Tf
9.843 0 0 9.843 69.7323 626.5132 Tm
-.0218 Tc
[(Ce)-6.8(lls)-333.7(w)-6.5(e)-1(re)-335.1(ha)-6.8(rve)-6.8(s).3(te)-6.8(d)-328.2(a)-6.8(fte)-6.8(r)-328.1(b)-5.6(r).2(i)-5.7(e)-1(f)-333.9(e)-1(xp)-5.6(osu)-5.6(r).2(e)-335.1(t)0(o)-333.9(0).1(.25)-5.6(%)-329.2(t)-5.7(r).2(yp)-5.6(si)-5.7(n/)]TJ
-1.0137 -1.1116 TD
-.0199 Tc
[(1)-170.7(m).8(M)-373.2(e)-4.9(thylenediaminetetraac)-4.9(et)]TJ
12.2336 0 TD
-.0202 Tc
[(ic)-373.8(a)-5.2(c).6(id)-378.4(\(N)-4.9(aca)-5.2(lai)-378.5(Tesque,)-375(Inc.\).)]TJ
-12.2336 -1.1174 TD
-.0217 Tc
[(A)-6.4(f).3(te)-6.7(r)-379.9(w)-.6(as)-5.3(hi)-5.6(ng)-379.9(wit)-5.6(h)-379.9(0.1%)-381(bo)-5.5(vin)-5.5(e)-381.1(ser)-5.5(u).2(m)-381.1(a)-.9(l)-5.6(bumi)-5.6(n)-379.9(in)-379.9(PB)-6.5(S,)-376.5(t)-5.6(h).2(e)]TJ
0 -1.1116 TD
-.0197 Tc
[(c)-4.7(e)1.1(lls)-573.5(w)1.4(e)-4.7(r)2.3(e)-574.9(t)2.1(reated)-573.7(w)-4.4(ith)-573.7(PMab-48)-573.7(\()2.3(10)]TJ
/F8 1 Tf
15.8104 0 TD
0 Tc
(m)Tj
/F3 1 Tf
.5299 0 TD
-.0216 Tc
[(g/)-5.5(mL\))-575.6(o)-5.4(r)-575.6(P).5(M)-6.3(a)-.8(b-)-5.4(48)]TJ
-16.3403 -1.1116 TD
-.0162 Tc
[(\(1)5.7(0)]TJ
/F8 1 Tf
1.4399 0 TD
0 Tc
(m)Tj
/F3 1 Tf
.5357 0 TD
-.0216 Tc
[(g/)-5.5(mL\))-402.8(p).3(l)-5.5(u).3(s)-402.6(p).3(e)-6.6(p).3(t)-5.5(i).2(de)-6.6(s)-402.6(\().4(10)]TJ
/F8 1 Tf
9.8261 0 TD
0 Tc
(m)Tj
/F3 1 Tf
.5299 0 TD
-.0215 Tc
[(g/)-5.4(mL\))-402.7(f)-5.3(or)-402.7(30)-402.7(mi)-5.4(nu)-5.3(tes)-402.5(a)-6.5(t)-402.9(4)]TJ
/F9 1 Tf
10.69 0 TD
0 Tc
()Tj
/F3 1 Tf
.3744 0 TD
-.0209 Tc
(C,)Tj
-23.3959 -1.1116 TD
-.0202 Tc
[(follow)-4.9(ed)-361.1(by)-361.1(treatment)-361.2(with)-361.1(Alex)]TJ
12.9939 0 TD
-.0198 Tc
[(a)-361.9(F)2.3(luor)-360.7(488-conjugated)-360.7(anti-)]TJ
-12.9939 -1.1174 TD
-.0202 Tc
[(m)-5.2(ouse)-264.4(I)1.8(gG)-264.1(\(1:1000;)-263.3(Cell)-263.3(Signa)-5.2(lin)]TJ
13.0803 0 TD
[(g)-263.1(Technology,)-259.7(Inc)-5.1(.)5.3(,)-265.4(D)1(a)-5.1(nve)-5.1(rs,)]TJ
-13.0803 -1.1116 TD
-.0216 Tc
[(M)-6.3(A)-.5(\).)-313(F)-5.3(l).2(uo)-5.4(res)-5.2(c)-.8(e)-6.6(n).3(ce)-311.8(d)-5.4(a)-.8(ta)-317.6(wer)-5.4(e)-311.8(ac)-6.6(qui)-5.5(red)-316.4(u).3(si)-5.5(ng)-310.7(t)-5.5(h).3(e)-311.8(c)-6.6(ell)-316.6(a)-.8(na)-6.6(lyz)-6.6(e)-.8(r)]TJ
T*
[(S)-5.4(A)-.6(38)-5.5(00)-282(\()-5.5(S).4(on)-5.5(y)-282(C)-.8(or)-5.5(p.,)-284.3(T)-1(oky)-5.5(o,)-284.3(Ja)-6.7(pan)-5.5(\)).3(.)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 59.7543 511.4266 Tm
0 Tc
(Results)Tj
/F3 1 Tf
9.843 0 0 9.843 69.7323 494.9857 Tm
[(We)-293.6(previously)-294.4(developed)-295.8(a)-296(novel)-294.8(PMab-48)-291.8(mAb)-293.4(that)-293.8(ex-)]TJ
-1.0137 -1.1116 TD
[(hibits)-323.6(high)-327.1(specicity)-325.6(and)-321.2(sensitivity)-330.3(against)-326.9(dPDPN)-323.7(and)-321.2(is)]TJ
T*
[(highly)-225.7(suitable)-230.2(for)-227.9(detection)-226.9(of)-226.8(lymphatic)-227(endothelial)-229.2(cells)-226.8(in)]TJ
0 -1.1174 TD
[(immunohistochemical)-335(analysis.)]TJ
7.3823 0 0 6.5614 185.1023 466.5258 Tm
(\(10\))Tj
9.843 0 0 9.843 200.6362 462.1038 Tm
[(PMab-48)-326.4(can)-328.2(be)-325.9(used)-327.9(to)]TJ
-14.3129 -1.1116 TD
[(detect)-396.3(dPDPN)-398.6(expressed)-398.9(by)-393.9(squamous)-391(cell)-398.6(carcinomas.)]TJ
7.3823 0 0 6.5614 286.5259 455.5274 Tm
(\(10\))Tj
9.843 0 0 9.843 59.7543 440.2204 Tm
[(These)-256.5(ndings)-249.4(suggest)-250.5(that)-253.5(PMab-48)-251.5(epitope)-252.2(mapping)-252.1(could)]TJ
T*
[(be)-297.1(a)-296(promising)-299.2(target)-297.1(for)-297(PDPN-based)-302.5(diagnosis)-293.2(and)-298.2(cancer)]TJ
0 -1.1174 TD
(therapy.)Tj
1.0137 -1.1116 TD
[(In)-439.9(this)-436.4(study,)-435.1(we)-442(synthesized)-441.7(a)-440(series)-440.6(of)-434.1(point)-437.7(mutants)]TJ
-1.0137 -1.1116 TD
[(of)-416.9(dPDPN)-415.9(peptides)-414.4(from)-414.5(the)-413.5(26th)-413.5(to)-417(the)-413.5(40th)-413.5(amino)-417(acid)]TJ
0 -1.1174 TD
[(\(Table)-396.1(1\).)-385.7(Using)-392.2(ELISA,)-386.4(PMab-48)-389.7(detected)-393.9(V26A,)-389.7(R27A,)]TJ
0 -1.1116 TD
[(P28A,)-221.7(G34A,)-222.7(V35A,)-216.9(E36A,)-223(D37A,)-216.9(S38A,)-221.7(V39A,)-216.9(and)-223.3(V40A;)]TJ
T*
[(in)-267.3(contrast,)-268(PMab-48)-268.8(did)-262.6(not)-268.4(react)-268.3(with)-264.7(D29A,)-268.8(D30A,)-263(I31A,)]TJ
0 -1.1174 TD
[(I32)14(A,)-293.4(or)-290.2(P33)14.1(A,)-287.6(ind)13.8(ica)11.5(tin)13.7(g)-289.1(tha)12.7(t)-295(the)-286.8(DDI)12.3(IP)-290(seq)13(uen)12.8(ce)-286.7(is)-290.1(a)-296(cri)12.7(t-)]TJ
0 -1.1116 TD
[(ica)11.5(l)-277.7(epi)12.7(top)13.8(e)-273(o)0(f)-272.9(PMa)17.8(b-4)14(8.)]TJ
1.0137 -1.1116 TD
.0188 Tc
[(Ne)5(x)6.2(t).3(,)-468.4(w)-.4(e)-467.3(p).4(e)5(r)6.2(fo)6.2(rm)5(ed)-466.1(a)-473(b)6.2(lo)6.2(ck)6.2(i)6.1(n).4(g)-471.9(a)5(ss)6.4(a)5(y)-471.9(us)6.4(in)6.2(g)-471.9()6.3(ow)-467(cy)6.2(-)]TJ
-1.0137 -1.1116 TD
.0172 Tc
[(to)4.6(metr)4.6(y)4.6(.)-314.5(PMab-)4.6(4)-1.2(8)-312.2(r)4.6(eact)4.5(ed)-312.2(wi)4.5(th)-312.2(t)4.5(h)-1.2(e)-313.4(C)3.6(HO/)4.5(d)-1.2(P)4.7(D)-2(P)4.8(N)-313.1(cell)-312.3(l)4.5(i)-1.3(n)4.6(e)]TJ
25.5097 -21.1554 TD
.013 Tc
[(\(F)-5.2(ig)-5.4(.)-353.3(1).4(\))-5.3(.)-353.3(T)-6.6(h).4(is)-356.5(r)-5.3(e)-.8(a)-6.5(c)-.8(ti)-5.5(on)-356.7(w)-6.2(a)-.8(s)-356.5(c)-.8(o)-5.4(m)-.8(pl)-5.5(et)-5.5(el)-5.5(y)-351(n)-5.4(eu)-5.4(tr)-5.3(ali)-5.5(z)-.8(e)-6.5(d)-351(b)-5.4(y)-356.7(th)-5.4(e)]TJ
0 -1.1174 TD
[(wi)-5.4(ld)-5.3(-t)-5.4(yp)-5.3(e)-375.1(p)-5.3(ep)-5.3(ti)-5.4(de)-380.8(\(V)-6.1(RP)-5.1(DDI)-5.2(IP)-5.1(GV)-6.1(ED)-6.1(SV)-6.1(V\))-379.6(an)-5.3(d)-373.9(V)-6.1(35)-5.3(A;)-379.8(in)]TJ
0 -1.1116 TD
[(co)-5.5(nt)-5.6(ra)-6.6(st)-5.6(,)-186.4(D)-.6(2)-5.5(9).3(A)-6.3(,)-186.4(D3)-5.5(0A)-6.3(,)-186.4(I)-5.4(31)-5.5(A,)-186.4(I)-5.4(3).3(2)-5.5(A)-.6(,)-192.1(a)-.9(nd)-189.8(P)-5.3(3).3(3)-5.5(A)-184.9(d)-5.5(i).2(d)-189.8(n).3(o)-5.5(t)-189.9(bl)-5.6(ock)]TJ
T*
.018 Tc
[(t)5.3(h)-.4(e)-519.9(r)5.4(eact)5.3(io)5.4(n)-518.7(o)-.4(f)-518.7(P)5.5(Mab-)5.4(48)-518.8(wit)5.3(h)-518.7(CHO/)5.3(dP)5.5(DP)5.5(N,)-515.3(in)5.4(di)5.3(cati)5.3(n)5.4(g)]TJ
0 -1.1174 TD
.0151 Tc
[(that)-343.2(the)-344.3(D)-4.1(DIIP)-342.9(sequence)-344.3(is)-342.9(a)-344.3(c)1.3(ritic)-4.4(a)1.3(l)-343.2(e)-4.4(pitope)-344.3(of)-343.1(PM)-4.2(ab-48)]TJ
0 -1.1116 TD
.0126 Tc
[(\(F)-5.6(ig)-5.8(.)-278.8(2)-5.8(\).)]TJ
ET
q
1 i
311.414 732.245 m
549.354 732.245 l
549.354 209.707 l
311.414 209.707 l
W n
q
238.023 0 0 522.539 311.414 209.707 cm
/Im1 Do
Q
Q
BT
/F4 1 Tf
9.845 0 0 9.845 310.8472 192.9259 Tm
0 Tc
[(FIG.)-256.1(1.)]TJ
/F3 1 Tf
4.0367 0 TD
[(Flow)-258.2(cytometry)-266.1(using)-258.3(PMab-48)-256.4(and)-263.3(point)-258.7(mutants)]TJ
-4.0367 -1.0135 TD
[(of)-393.6(dPDPN.)-395.4(PMab-48)-400.3(or)-393.6(PMab-48)]TJ
/F9 1 Tf
14.3733 0 TD
(+)Tj
/F3 1 Tf
.9444 0 TD
[(peptides)-396.3(\(WT,)-401.2(D29A,)]TJ
-15.3177 -1.0135 TD
[(D30A,)-550.3(I31A,)-549.5(I32A,)-555.2(P33A,)-549.3(or)-549(V35A\))-553.6(were)-554.2(treated)-552.1(with)]TJ
0 -1.0077 TD
[(CHO/dPDPN)-370(cells)-364.5(for)-365.8(30)-364.8(minutes)-366.5(at)-366.1(4)]TJ
/F9 1 Tf
16.0318 0 TD
()Tj
/F3 1 Tf
.3973 0 TD
[(C,)-368.1(followed)-367.3(by)-364.8(ad-)]TJ
-16.4291 -1.0135 TD
[(dition)-513.2(of)-514.5(secondary)-519(antibodies.)-517(dPDPN,)-516.4(dog)-515.5(podoplanin;)]TJ
T*
[(WT,)-331.2(wild)-339.1(type;)-334.7(gray)-333.4(peak,)-332.2(negative)-340(control.)]TJ
ET
59.754 264.302 239.131 .22675 re
f
59.754 77.386 239.131 .28342 re
f
59.754 245.594 239.131 .28342 re
f
BT
/F6 1 Tf
9.845 0 0 9.845 79.937 279.9495 Tm
(Table)Tj
/F3 1 Tf
2.9944 0 TD
(1.)Tj
/F6 1 Tf
1.2496 0 TD
[(Determination)-339.5(of)-331.2(PMab-)]TJ
/F3 1 Tf
11.2752 0 TD
(48)Tj
/F6 1 Tf
1.3302 0 TD
(Epitope)Tj
-16.0491 -1.0135 TD
[(by)-330(Enzyme-Linked)-340.5(Immunosorbent)-334.5(Assay)]TJ
/F10 1 Tf
-2.8505 -1.8427 TD
[(Mutation)-6718.1(Sequence)-6324(PMab-48)]TJ
/F3 1 Tf
0 -1.8946 TD
[(Wild)-334.7(type)-3351(VRPDDIIPGVEDSVV)]TJ
/F9 1 Tf
21.5715 0 TD
(+++)Tj
/F3 1 Tf
-21.5715 -1.0135 TD
[(V26A)-4986.7(ARPDDIIPGVEDSVV)]TJ
/F9 1 Tf
21.8479 0 TD
(++)Tj
/F3 1 Tf
-21.8479 -1.0135 TD
[(R27A)-5038.7(VAPDDIIPGVEDSVV)]TJ
/F9 1 Tf
22.1243 0 TD
(+)Tj
/F3 1 Tf
-22.1243 -1.0077 TD
[(P28A)-5152.7(VRADDIIPGVEDSVV)]TJ
/F9 1 Tf
21.5715 0 TD
(+++)Tj
/F3 1 Tf
-21.5715 -1.0135 TD
[(D29A)-4986.7(VRPADIIPGVEDSVV)-5130.9()]TJ
T*
[(D30A)-4986.7(VRPDAIIPGVEDSVV)-5130.9()]TJ
T*
[(I31A)-5371.7(VRPDDAIPGVEDSVV)-4745.9()]TJ
0 -1.0077 TD
[(I32A)-5371.7(VRPDDIAPGVEDSVV)-4745.9()]TJ
0 -1.0135 TD
[(P33A)-5152.7(VRPDDIIAGVEDSVV)-4964.9()]TJ
T*
[(G34A)-4986.7(VRPDDIIPAVEDSVV)]TJ
/F9 1 Tf
21.8479 0 TD
(++)Tj
/F3 1 Tf
-21.8479 -1.0135 TD
[(V35A)-4986.7(VRPDDIIPGAEDSVV)]TJ
/F9 1 Tf
21.5715 0 TD
(+++)Tj
/F3 1 Tf
-21.5715 -1.0077 TD
[(E36A)-5090.7(VRPDDIIPGVADSVV)]TJ
/F9 1 Tf
21.5715 0 TD
(+++)Tj
/F3 1 Tf
-21.5715 -1.0135 TD
[(D37A)-4986.7(VRPDDIIPGVEASVV)]TJ
/F9 1 Tf
21.5715 0 TD
(+++)Tj
/F3 1 Tf
-21.5715 -1.0135 TD
[(S38A)-5152.7(VRPDDIIPGVEDAVV)]TJ
/F9 1 Tf
22.1243 0 TD
(+)Tj
/F3 1 Tf
-22.1243 -1.0135 TD
[(V39A)-4986.7(VRPDDIIPGVEDSAV)]TJ
/F9 1 Tf
21.5715 0 TD
(+++)Tj
/F3 1 Tf
-21.5715 -1.0077 TD
[(V40A)-4986.7(VRPDDIIPGVEDSVA)]TJ
/F9 1 Tf
21.5715 0 TD
(+++)Tj
8.7511 0 0 8.7511 67.748 65.5937 Tm
.0116 Tc
[(++)11.6(+)]TJ
/F3 1 Tf
1.6844 0 TD
.4043 Tc
[(,O)396.7(D)390.2(6)404.3(5)387.2(5)]TJ
/F11 1 Tf
3.8028 0 TD
0 Tc
(S)Tj
/F3 1 Tf
1.1791 0 TD
.0105 Tc
[(0.6)10.5(;)]TJ
/F9 1 Tf
1.9629 0 TD
.0116 Tc
(++)Tj
/F3 1 Tf
1.1208 0 TD
.4043 Tc
[(,0)392.5(.)395.2(4)]TJ
/F11 1 Tf
2.099 0 TD
0 Tc
(&)Tj
/F3 1 Tf
1.1791 0 TD
[(O)-7.6(D)-14.1(65)-17.1(5)]TJ
/F9 1 Tf
3.1485 0 TD
(<)Tj
/F3 1 Tf
.7321 0 TD
.0105 Tc
[(0.6)10.5(;)]TJ
/F9 1 Tf
1.9629 0 TD
0 Tc
(+)Tj
/F3 1 Tf
.5636 0 TD
.4043 Tc
[(,0)404.3(.)389.9(2)]TJ
/F11 1 Tf
2.099 0 TD
0 Tc
(&)Tj
/F3 1 Tf
1.1726 0 TD
.013 Tc
[(OD)5.4(65)13(5)]TJ
/F9 1 Tf
3.155 0 TD
0 Tc
(<)Tj
/F3 1 Tf
-26.7751 -1.0236 TD
[(0.)-14.5(4)-11.8(;)-347.4(,)-350.2(O)-7.6(D)-14.1(65)-17.1(5)]TJ
/F9 1 Tf
6.6533 0 TD
(<)Tj
/F3 1 Tf
.7321 0 TD
[(0.)-20.9(2.)]TJ
/F5 1 Tf
8.9663 0 0 8.9663 59.7543 749.7069 Tm
[(EPITOPE)-339(OF)-331.2(PM)]TJ
6.4556 0 0 6.4556 130.6771 749.7069 Tm
(AB)Tj
8.9663 0 0 8.9663 139.9181 749.7069 Tm
(-48)Tj
44.0707 0 TD
(163)Tj
ET
endstream
endobj
20 0 obj
<>stream
Adobe d
A
s !1AQa"q2B#R3b$r%C4Scs5D'6Tdt&
EFVU(eufv7GWgw8HXhx)9IYiy*:JZjz m !1AQa"q2#BRbr3$4CS%cs5DT
&6E'dtU7()euFVfvGWgw8HXhx9IYiy*:JZjz ? N*UثWb]v*UثWb.o?˨ndVT'1r24똇]DHc؋?Zg`yGI,KAipW ra ;Mغ%
-G/0g]+~E~Zcj}Oͳh4GhF59-.$jiNj>