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[(w)-3.8(e)1.6(re)-701(wa)-4.1(shed)-699.9(w)-3.8(i)2.7(th)-699.9(Dulbec)-4.1(co)]TJ
12.1299 0 TD
-.0259 Tc
[(s)-706.4(modied)-706.6(Eagle)-5.1(s)-706.4(medium)]TJ
-12.1299 -1.1116 TD
[(\(DM)-4.8(E).7(M;)-205.6(Na)-5(ca)-5(la)-5(i)-199.8(T)-5(esque,)-207.8(Inc.\))-205.4(a)]TJ
12.4122 0 TD
-.0274 Tc
[(n)-5.5(d)-207(re)-6.6(su)-5.5(sp)-5.5(en)-5.5(de)-6.6(d)-201.3(i)-5.6(n)-201.3(D)-6.3(ME)-6.7(M)-202.2(w)-6.3(it)-5.6(h)]TJ
-12.4122 -1.1116 TD
-.0259 Tc
[(10%)-344.8(F)2(B)-5(S)-337.9(to)-338(be)-339.2(used)-343.8(as)-343.6(effe)-5.1(ctor)-343.7(ce)-5.1(lls.)-346.1(SN)-4.8(P)-337.9(c)-5.1(ells)-343.6(we)-5.1(re)-344.9(la)-5.1(be)-5.1(le)-5.1(d)]TJ
0 -1.1174 TD
-.0215 Tc
[(wi)6.1(th)-224.2(1)6.2(0)]TJ
/F11 1 Tf
3.0238 0 TD
0 Tc
(l)Tj
/F4 1 Tf
.5299 0 TD
-.0249 Tc
[(g/mL)-228.8(C)-4(a)1.6(lc)-4.1(ein)-227.6(A)1.9(M)-228.5(\()-2.9(Thermo)-227.6(Fisher)-227.6(Scientic)-4.1(,)-224.2(Inc)-4.1(.).5(\))-227.6(a)1.6(nd)]TJ
-3.5537 -1.1116 TD
-.0232 Tc
[(resu)4.5(sp)4.5(en)4.5(ded)-277.7(i)-1.4(n)-277.7(t)4.4(he)-278.9(s)4.7(a)-2.4(me)-278.9(med)4.5(i)-1.4(u)4.5(m)-2.5(.)]TJ
1.0137 -1.1116 TD
0 Tc
[(Th)19.6(e)-307.5(t)0(a)19.5(r)0(g)20.9(e)0(t)-303(c)0(e)18.5(l)0(l)20.6(s)-306.2(\(2)]TJ
/F9 1 Tf
7.4876 0 TD
()Tj
/F4 1 Tf
.7084 0 TD
(10)Tj
7.3823 0 0 6.5614 162.3118 587.0549 Tm
(4)Tj
9.843 0 0 9.843 169.0582 582.6896 Tm
[(ce)18.5(ll)20.6(s/)15.1(we)18.8(ll)20.6(\))-306.3(w)0(e)18.8(r)0(e)-297.1(p)0(l)15(a)0(t)19.5(e)0(d)-297.1(i)0(n)-296.1(9)0(6)20.8(-)0(w)14.2(e)0(l)19.5(l)]TJ
-10.8743 -1.1116 TD
[(pl)20.7(at)19.5(es)-216.3(an)19.7(d)-220(m)0(i)19.5(x)0(e)19.7(d)-220(wi)19.8(th)-209.7(ef)19.7(fe)19.7(ct)13.8(or)-209.5(ce)18.5(ll)20.6(s)-219.8(\()0(e)19.7(f)0(f)15.1(e)0(c)18.5(t)0(o)20.7(r)0(/)20.7(t)0(a)19.6(r)0(g)20.9(e)0(t)-210.8(c)0(e)18.5(l)0(l)14.8(s)-219.8(ra)19.7(ti)20.6(o,)]TJ
0 -1.1174 TD
[(50)20.8(\),)-327(10)20.8(0)]TJ
/F11 1 Tf
3.5249 0 TD
(l)Tj
/F4 1 Tf
.5414 0 TD
[(g/)20.7(mL)-327.2(of)-330.5(13)20.8(4-)20.9(mG)]TJ
7.3823 0 0 6.5614 170.1354 559.1053 Tm
-.0162 Tc
(2a)Tj
9.843 0 0 9.843 176.9385 560.7495 Tm
0 Tc
[(-f)-324.7(or)-330.5(co)19.7(nt)20.7(ro)20.9(l)-335.3(m)0(o)19.6(u)0(s)21(e)-336.3(Ig)15.1(G)]TJ
7.3823 0 0 6.5614 272.4661 559.1053 Tm
-.0162 Tc
(2a)Tj
9.843 0 0 9.843 279.2692 560.7495 Tm
.3317 Tc
[(.F)331.7(o)346.9(l)331.7(-)]TJ
-22.0712 -1.1116 TD
0 Tc
[(lo)20.7(wi)19.8(ng)-186.5(4.)18.5(5-)20.9(ho)20.8(ur)-186.5(in)15(cu)19.7(ba)19.7(ti)20.6(on)-186.5(at)-187.8(37)]TJ
/F9 1 Tf
12.7117 0 TD
()Tj
/F4 1 Tf
.3859 0 TD
[(C,)-184.1(th)20.7(e)-198.1(r)0(e)19.7(l)0(e)19.6(a)0(s)14.1(e)-192.3(of)-186.5(Ca)18.6(lc)13.8(ei)19.5(n)-196.9(i)0(n)20.7(t)0(o)]TJ
-13.0976 -1.1116 TD
[(th)20.7(e)-284.5(s)0(u)15.3(p)0(e)19.7(r)0(n)20.9(a)0(t)19.5(a)0(n)19.7(t)-283.5(wa)18.8(s)-288.9(m)0(e)18.4(a)0(s)19.9(u)0(r)20.9(e)0(d)-274.1(i)0(n)-278.8(e)0(a)18.5(c)0(h)-274.1(w)0(e)18.8(l)0(l)20.6(.)-285.7(Th)19.6(e)-284.5()0(u)21(o)0(r)20.9(e)0(s)14.1(c)0(e)18.5(n)0(c)19.7(e)]TJ
0 -1.1174 TD
[(in)20.7(te)19.6(ns)15.3(it)20.6(y)-410(w)0(a)18.8(s)-409.8(de)19.7(te)19.5(rm)19.6(in)20.7(ed)-400.8(us)15.3(in)20.7(g)-410(a)-411.2(mi)19.5(cr)19.7(op)20.8(la)19.6(te)-400.9(re)19.7(ad)19.7(er)-400.7(\(P)15.3(ow)20(er)]TJ
0 -1.1116 TD
[(Sc)19.8(an)-343.2(HT)18.8(;)-352.6(B)0(i)19.7(o)0(T)19.6(e)0(k)-343.2(I)0(n)20.9(s)0(t)20.9(r)0(u)20.9(m)0(e)18.4(n)0(t)20.7(s)0(,)-344.2(I)0(n)20.9(c)0(.)17.3(,)-349(Wi)18.4(no)20.8(os)21(ki)14.9(,)-349(V)0(T)18.8(\))-352.4(wi)19.8(th)-342.1(an)]TJ
T*
[(ex)19.7(ci)19.5(ta)19.5(ti)14.8(on)-238.3(wa)18.8(ve)19.7(le)19.5(ng)20.8(th)-244.2(of)-238.3(48)20.8(5)-156.6(n)0(m)-245.3(a)0(n)19.7(d)-248.8(an)-239.5(em)18.5(is)15.1(si)20.9(on)-238.3(wa)18.8(ve)19.7(le)13.8(ng)20.8(th)]TJ
0 -1.1174 TD
[(of)-290.2(53)20.8(8)-156.6(n)0(m)19.6(.)-297.2(Cy)19.8(to)20.7(ly)20.7(ti)20.6(c)-296(a)0(c)12.8(t)0(i)20.6(v)0(i)20.7(t)0(y)-284.6(\()0(%)-291.2(l)0(y)20.7(s)0(i)20.9(s)0(\))-290(w)0(a)18.8(s)-294.6(ca)18.5(lc)19.6(ul)20.7(at)13.8(ed)-285.6(as)-285.4(fo)15.1(l-)]TJ
0 -1.1116 TD
[(lo)20.7(ws)20.2(:)-410.2(%)-405.3(ly)20.7(si)15.1(s)]TJ
/F9 1 Tf
5.7251 0 TD
(=)Tj
/F4 1 Tf
.7084 0 TD
(\(E)Tj
/F9 1 Tf
.9273 0 TD
(-)Tj
/F4 1 Tf
.5414 0 TD
[(S\))21(/\()-65.7(M)]TJ
/F9 1 Tf
2.4191 0 TD
(-)Tj
/F4 1 Tf
.5414 0 TD
(S\))Tj
/F9 1 Tf
1.031 0 TD
()Tj
/F4 1 Tf
.7084 0 TD
[(10)20.8(0,)-396.2(wh)14.2(er)19.7(e)-405.4()62.3(E)13.9()62.3()-404.2(is)-399.5(th)20.7(e)-405.4()0(u)15.2(o)0(-)]TJ
-12.6022 -1.1116 TD
[(re)19.7(sc)19.9(en)13.9(ce)-217.6(me)18.4(as)19.9(ur)20.9(ed)-216.5(in)-221.2(co)19.7(mb)19.6(in)20.7(ed)-216.5(cu)19.7(lt)20.6(ur)15.1(es)-216.3(of)-215.3(ta)19.6(rg)15.1(et)-216.6(an)19.7(d)-225.7(e)0(f)19.7(f)0(e)14(c)0(t)19.5(o)0(r)]TJ
0 -1.1174 TD
[(ce)18.5(ll)20.6(s,)-217.4()62.3()0(S)21()56.5()-225.7(i)0(s)-215.2(t)0(h)20.7(e)-226.9(sp)21(on)20.8(ta)19.6(ne)19.7(ou)20.8(s)-225.5()0(u)21(o)0(r)15.1(e)0(s)19.9(c)0(e)18.5(n)0(c)19.7(e)-226.9(of)-215.3(ta)19.6(rg)20.9(et)-216.6(ce)18.5(ll)20.6(s)-225.5(o)0(n)20.8(l)0(y)20.7(,)]TJ
0 -1.1116 TD
[(an)19.7(d)-346.7()62.3(M)20()56.5()-340.9(is)-336.2(th)20.7(e)-347.8(m)15(ax)13.9(im)19.5(um)-331.7(u)21(or)20.9(es)14.1(ce)18.5(nc)19.7(e)-342.1(m)0(e)18.4(a)0(s)19.9(u)0(r)15.1(e)0(d)-331.7(f)0(o)20.9(l)0(l)20.6(o)0(w)14.2(i)0(n)20.7(g)]TJ
T*
[(th)20.7(e)-445.8(l)0(y)20.7(s)0(i)20.9(s)-444.4(of)-428.4(al)19.5(l)-444.7(c)0(e)18.5(l)0(l)20.6(s)-438.6(wi)14.1(th)-428.5(a)-440(b)0(u)15.1(f)0(f)20.9(e)0(r)-429.5(c)0(o)19.7(n)0(t)14.9(a)0(i)19.5(n)0(i)20.7(n)0(g)-428.4(0)0(.)18.5(5)0(%)-429.5(T)0(r)13.9(i)0(t)20.6(o)0(n)]TJ
T*
[(X-)20(10)20.8(0,)-263.7(10)-146.2(mM)-257.8(Tr)19.7(is)15.1(-H)20(Cl)-262.6(\(p)20.9(H)-266.9(7)0(.)18.5(4)0(\))20.9(,)-274.1(an)19.7(d)-266(1)0(0)-146.2(m)0(M)-263.5(o)0(f)-261.4(E)0(D)18.8(T)0(A)18.8(.)]TJ
/F10 1 Tf
8.9663 0 0 8.9663 62.022 405.4676 Tm
[(Complement-dependent)-335.2(cytotoxicity)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 389.0266 Tm
[(S)16.3(N)0(P)-164.2(c)15(el)25.3(ls)-163.4(we)24.6(r)16.2(e)-180.8(l)16.1(a)15(be)25.4(le)25.3(d)-173.9(w)0(i)25.6(t)16.1(h)-179.6(1)16.2(0)]TJ
/F11 1 Tf
12.0723 0 TD
(l)Tj
/F4 1 Tf
.5357 0 TD
[(g)16.2(/)0(m)25.2(L)-175.1(Ca)24.4(l)16.1(c)0(e)24.3(i)16.1(n)-179.6(A)15.3(M)-180.5(\()16.2(Th)25.4(e)15(r)0(m)25.4(o)]TJ
-13.6217 -1.1116 TD
[(F)16.3(i)0(s)26.7(h)0(e)25.4(r)-306.3(Sc)25.6(ie)25.3(n)16.2(t)0(i)26.3()16.3(c,)-299.4(I)16.2(n)16.2(c)0(.)23.1(\))-306.3(a)15(n)0(d)-295.9(r)0(e)25.5(s)16.4(us)26.8(pe)25.4(n)16.2(d)0(e)25.4(d)-306.4(in)-296.1(t)16.1(h)0(e)-297.1(m)15(ed)25.4(i)16.1(u)0(m)25.4(.)-308.7(T)15(h)16.2(ey)]TJ
T*
[(w)15.3(e)0(r)25.5(e)-382.4(t)16.1(h)16.2(en)-366.2(pl)26.5(a)15(t)0(e)25.3(d)-381.2(i)16.1(n)-375.5(96)26.6(-w)25.8(e)15(l)0(l)-365.3(p)0(l)26.5(a)15(te)25.3(s)-381(a)15(t)-375.6(2)]TJ
/F9 1 Tf
16.0177 0 TD
()Tj
/F4 1 Tf
.7027 0 TD
(10)Tj
7.3823 0 0 6.5614 236.1826 371.5085 Tm
(4)Tj
9.843 0 0 9.843 243.6094 367.1432 Tm
-.0154 Tc
[(cel)-15.4(l)10.9(s)-15.4(/)11.3(w)-.1(e)-15.4(l)9.9(l)-396.8(w)-.1(it)-15.4(h)]TJ
-18.4483 -1.1174 TD
0 Tc
[(ra)25.5(b)16.2(b)0(i)26.5(t)-433.2(c)15(o)16.2(mp)25.4(l)16.1(e)0(m)24.2(e)15(nt)-417(\()26.8(na)25.4(l)-433.2(d)16.2(il)26.3(u)16.2(t)0(i)26.3(o)16.2(n)-433.1(1)0(:)26.5(1)16.2(0;)-417(Lo)25.4(w)15.3(-)0(T)25.4(o)0(x)26.6(-)16.2(M)-434(R)15.2(ab)25.4(b)16.2(i)0(t)]TJ
0 -1.1116 TD
[(C)15.2(o)0(m)25.4(p)16.2(le)25.3(m)15(e)0(n)25.4(t)16.1(;)-404.4(C)15.2(e)15(d)0(a)25.4(r)16.2(la)25.3(n)16.2(e)-399.7(La)24.2(b)16.2(o)0(r)26.6(a)0(t)25.3(o)16.2(ri)26.5(e)15(s)0(,)-390.2(H)15.3(o)16.2(r)0(n)26.6(b)0(y)26.6(,)-400.8(O)15.3(n)16.2(ta)25.3(ri)26.5(o)16.2(,)-400.8(C)15.2(a)0(-)]TJ
T*
-.0162 Tc
[(na)-16.2(d)9.2(a)-1.2(\))-322.5(a)-1.2(nd)-322.6(100)]TJ
/F11 1 Tf
5.8231 0 TD
0 Tc
(l)Tj
/F4 1 Tf
.5357 0 TD
[(g/)26.5(m)15(L)-301.8(of)-290.2(1)16.2(3)0(4)26.6(-)16.2(mG)]TJ
7.3823 0 0 6.5614 191.622 332.6172 Tm
-.0162 Tc
(2a)Tj
9.843 0 0 9.843 198.3684 334.2613 Tm
0 Tc
[(-)16.2(f)-300.6(or)-290.2(c)15(o)0(n)26.6(t)0(r)26.5(o)16.2(l)-306.5(m)15(o)16.2(u)0(s)26.8(e)-301.8(Ig)26.6(G)]TJ
7.3823 0 0 6.5614 291.9117 332.6172 Tm
-.0162 Tc
(2a)Tj
9.843 0 0 9.843 298.6582 334.2613 Tm
0 Tc
(.)Tj
-24.041 -1.1174 TD
[(F)16.3(o)0(l)26.5(l)0(o)26.5(w)15.3(in)26.5(g)-202.7(4)-208.4(h)16.2(o)16.2(u)0(r)26.6(s)-208.3(o)16.2(f)-202.7(in)26.5(c)15(u)0(b)26.6(a)15(ti)26.3(o)16.2(n)-208.4(a)15(t)-202.8(37)]TJ
/F9 1 Tf
14.5087 0 TD
()Tj
/F4 1 Tf
.3801 0 TD
[(C,)-195.6(w)15.3(e)-203.8(m)15(e)0(a)24.3(s)16.4(ur)26.6(e)15(d)-208.4(C)15.1(a)15(lc)25.3(e)15(i)0(n)]TJ
-14.8888 -1.1116 TD
[(re)25.5(l)16.1(e)0(a)24.3(s)16.4(e)-417(i)16.1(n)16.2(t)0(o)-399.7(t)16.1(he)-400.8(s)16.4(u)0(p)26.6(e)15(rn)26.6(at)25.3(a)15(n)0(t)-399.7(f)16.2(or)-399.6(e)15(a)0(c)24.3(h)-410(we)24.6(l)16.1(l)0(.)-402.1(F)16.3(l)16.1(u)0(o)26.6(r)0(e)25.5(s)16.4(ce)24.3(n)16.2(c)0(e)-401.9(i)16.1(n-)]TJ
T*
[(t)16.1(e)0(n)25.4(s)0(i)26.7(t)16.1(y)-185.4(w)15.3(a)15(s)-185.2(c)15(a)0(l)25.3(c)15(ul)26.5(a)15(t)0(e)25.3(d)-179.6(as)-170.2(de)25.4(s)16.4(c)0(r)25.5(i)0(b)26.5(e)15(d)-185.4(i)16.1(n)-179.6(t)0(h)26.5(e)-186.6(A)15.3(D)15.3(C)0(C)-171.3(s)16.4(ec)24.3(t)16.1(i)0(o)26.5(n)-179.6(ab)25.4(ov)26.6(e)15(.)]TJ
/F10 1 Tf
8.9663 0 0 8.9663 62.022 277.6251 Tm
[(Antitumor)-335.1(activity)-332.8(of)-336.7(134-mG)]TJ
6.7246 0 0 5.9768 176.4283 276.0944 Tm
(2a)Tj
8.9663 0 0 8.9663 183.8551 277.6251 Tm
[(-f)-334.4(in)-336.5(xenografts)]TJ
-13.5879 -1.2266 TD
[(of)-336.7(SNP)-333.7(cells)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 250.1858 Tm
[(A)-405.1(total)-404.5(of)-399.6(16)-405.4(female)-403.1(BALB/c)-409.5(nude)-403(mice)-405.5(\(5)-405.3(weeks)-405.9(old,)]TJ
-1.0137 -1.1116 TD
[(weighing)-350.9(1417)-166.8(g\))-342(were)-347.4(purchased)-353(from)-345.4(Charles)-345(River)-347.6(La-)]TJ
T*
[(boratories,)-322(Inc.,)-316.6(and)-315.5(used)-316.4(in)-319.1(experiments)-322.1(once)-317.8(they)-316.7(reached)]TJ
0 -1.1174 TD
[(7)-283.3(weeks)-290.7(of)-284.4(age.)-285.5(SNP)-286.1(cells)-290.1(\(0.3)-168(mL)-286.8(of)-284.4(1.33)]TJ
/F9 1 Tf
17.3252 0 TD
()Tj
/F4 1 Tf
.72 0 TD
(10)Tj
7.3823 0 0 6.5614 249.4487 221.7259 Tm
(8)Tj
9.843 0 0 9.843 255.9684 217.3039 Tm
[(cells/mL)-290.2(in)]TJ
-19.704 -1.1116 TD
[(DMEM\))-608.4(were)-606.6(mixed)-607(with)-604.5(0.5)-166.9(mL)-603.6(BD)-603.1(Matrigel)-611.4(Matrix)]TJ
T*
[(Growth)-423.2(Factor)-423.6(Reduced)-421.2(\(BD)-419.9(Biosciences,)-424.3(San)-424.8(Jose,)-417.5(CA\);)]TJ
0 -1.1174 TD
(100)Tj
/F11 1 Tf
1.6703 0 TD
(l)Tj
/F4 1 Tf
.5472 0 TD
[(L)-382.4(o)0(f)-388.1(this)-378.8(suspension)-386.1(\(5)]TJ
/F9 1 Tf
9.8145 0 TD
()Tj
/F4 1 Tf
.72 0 TD
(10)Tj
7.3823 0 0 6.5614 197.348 188.844 Tm
(6)Tj
9.843 0 0 9.843 204.8314 184.422 Tm
[(cells\))-383.3(was)-385.3(injected)-387.1(sub-)]TJ
-14.5087 -1.1116 TD
[(cutaneously)-326.7(into)-321.4(the)-321.4(left)-328.3(anks)-323.1(of)-319(the)-327.1(mice.)-322.5(On)-319.9(day)-327(7)-317.9(post-)]TJ
T*
[(inoculation,)-346.7(100)]TJ
/F11 1 Tf
6.7849 0 TD
(l)Tj
/F4 1 Tf
.5529 0 TD
[(g)-340.9(o)0(f)-347.7(134-mG)]TJ
7.3823 0 0 6.5614 186.9165 160.8945 Tm
(2a)Tj
9.843 0 0 9.843 193.9464 162.5385 Tm
[(-f)-347.7(\()]TJ
/F8 1 Tf
1.3478 0 TD
(n)Tj
/F9 1 Tf
.6624 0 TD
(=)Tj
/F4 1 Tf
.72 0 TD
[(8\))-347.7(or)-342(control)-348.9(mouse)]TJ
-16.1329 -1.1116 TD
[(IgG)-395.8(\()]TJ
/F8 1 Tf
2.2808 0 TD
(n)Tj
/F9 1 Tf
.6681 0 TD
(=)Tj
/F4 1 Tf
.72 0 TD
[(8\))-393.8(in)-394(100)]TJ
/F12 1 Tf
4.0721 0 TD
(m)Tj
/F4 1 Tf
.5529 0 TD
[(L)-393.9(PBS)-395.7(were)-399.2(injected)-398.6(intraperitoneally.)]TJ
-8.294 -1.1174 TD
[(Additional)-238.2(antibody)-229.1(inoculations)-240.5(were)-232.2(performed)-238(on)-226.8(days)-235.8(11)]TJ
0 -1.1116 TD
[(and)-217.6(18.)-213(At)-216.3(25)-215.3(days)-218.5(following)-218.5(cell)-220.1(implantation,)-216.7(all)-217.8(mice)-215.4(were)]TJ
T*
[(euthanized)-505.5(by)-503.3(cervical)-503.3(dislocation.)-507.8(Tumor)-503.2(diameters)-504.2(and)]TJ
0 -1.1174 TD
[(volumes)-280.7(were)-284(determined)-286.7(as)-279.6(previously)-282.9(described.)]TJ
7.3823 0 0 6.5614 263.055 112.1385 Tm
(\(28\))Tj
/F10 1 Tf
8.9663 0 0 8.9663 62.022 83.9622 Tm
[(Statistical)-336.1(analyses)]TJ
/F4 1 Tf
9.843 0 0 9.843 72 67.5212 Tm
[(All)-482.5(data)-479.2(are)-483.6(expressed)-485.3(as)-481.2(mean)]TJ
/F9 1 Tf
13.7139 0 TD
()Tj
/F4 1 Tf
.72 0 TD
[(standard)-483.3(error)-478.9(of)-480.2(the)]TJ
-15.4475 -1.1116 TD
[(mean.)-362.7(Statistical)-367.4(analysis)-368.1(was)-362.2(conducted)-364.9(with)-362.6(Tukeys)-361.2(test)]TJ
25.504 67.9242 TD
[(for)-360.3(ADCC)-365.3(and)-361.6(CDC,)-361(and)-355.8(Welchs)]TJ
/F8 1 Tf
14.6354 0 TD
(t)Tj
/F4 1 Tf
.6451 0 TD
[(test)-357(for)-360.3(tumor)-363.9(weight.)]TJ
-15.2805 -1.1174 TD
[(Analysis)-350.5(of)-347.7(variance)-353.4(\(ANOVA\))-351.8(and)-344.3(Sidaks)-348.4(multiple)-351.5(com-)]TJ
0 -1.1116 TD
[(parisons)-315(tests)-311.8(were)-307.1(conducted)-313(for)-308.5(tumor)-312.1(volume)-308.6(and)-309.7(mouse)]TJ
T*
[(weight.)-496.2(All)-488.2(calculations)-496.3(were)-491.4(performed)-491.4(using)-489.3(GraphPad)]TJ
T*
[(Prism)-306(8)-306.4(\(GraphPad)-306.6(Software,)-303.3(Inc.\).)-306.1(A)]TJ
/F8 1 Tf
15.6894 0 TD
(p)Tj
/F4 1 Tf
.4953 0 TD
[(-value)-308.5(of)]TJ
/F9 1 Tf
3.9512 0 TD
(<)Tj
/F4 1 Tf
.5529 0 TD
[(0.05)-300.5(was)]TJ
-20.6889 -1.1174 TD
[(consid)-7.7(ered)-277.4(statistically)-289(signicant.)]TJ
/F6 1 Tf
8.9663 0 0 8.9663 313.0582 648.3967 Tm
(Results)Tj
/F10 1 Tf
0 -1.8336 TD
[(Fl)16.3(ow)-215.9(cy)19.9(to)17.3(me)19.2(tr)19.6(y)-227.1(a)0(n)22.8(a)0(l)17.6(y)0(s)19.9(i)0(s)-217.8(a)0(g)22.8(a)0(i)17.6(n)0(s)21.4(t)-231.2(c)13.1(a)0(n)16.5(i)0(n)23.9(e)-232(ma)19.2(mm)22(ar)18.8(y)-227.1(g)0(l)17.6(a)14.6(nd)]TJ
0 -1.2203 TD
[(tu)17.3(mo)19.2(r)-324.6(c)13.1(el)17.6(l)-325.8(l)0(i)18.7(n)0(e)22.8(,)-326(SN)18.2(P)-320.4(c)0(e)15(l)0(l)18.7(s)-322(u)14.5(s)0(i)16.2(n)0(g)-312.3(r)0(e)18.8(c)13.1(EM)19.4(ab)16.5(-1)18.8(3)14.6(4)]TJ
T*
[(an)16.5(d)-320.6(1)0(3)16.5(4)0(-)18.8(m)0(G)]TJ
6.7246 0 0 5.9768 362.4377 608.5416 Tm
(2a)Tj
8.9663 0 0 8.9663 369.6944 610.0723 Tm
(-f)Tj
/F4 1 Tf
9.843 0 0 9.843 323.0361 593.6313 Tm
[(In)-376.5(our)-371.9(previous)-378.5(study,)-377.5(an)-372(anti-hEGFR)-378.2(mAb,)-377.5(EMab-134,)]TJ
-1.0137 -1.1116 TD
[(recognized)-344.1(dEGFR-overexpressed)-344.8(CHO/dEGFR)-338.3(cells,)-339.6(indi-)]TJ
0 -1.1174 TD
[(cating)-435.4(that)-437.8(EMab-134)-432.5(crossreacts)-442.5(with)-431.8(dEGFR.)]TJ
7.3823 0 0 6.5614 508.8755 576.0565 Tm
(\(28\))Tj
9.843 0 0 9.843 525.4865 571.6912 Tm
[(In)-434.1(this)]TJ
-21.5816 -1.1116 TD
[(study,)-412.1(recEMab-13)-10.8(4)-410(also)-408.7(detected)-416.9(canine)-413.4(mammary)-416.9(gland)]TJ
T*
[(tumor)-340.9(cell)-335.3(line,)-331.8(SNP)-337.9(cells)-336.2(\(Fig.)-336.1(1A\).)-335.9(Similarly,)-338.6(the)-332.9(defuco-)]TJ
0 -1.1174 TD
[(sylated)-465.1(mouse)-462.7(IgG)]TJ
7.3823 0 0 6.5614 391.181 537.1652 Tm
(2a)Tj
9.843 0 0 9.843 402.7463 538.8093 Tm
[(type)-460.7(of)-462.9(EMab-134,)-464.7(134-mG)]TJ
7.3823 0 0 6.5614 519.0235 537.1652 Tm
(2a)Tj
9.843 0 0 9.843 525.9967 538.8093 Tm
[(-f,)-466.3(de-)]TJ
-21.6335 -1.1116 TD
[(tecte)-9.3(d)-214.2(SNP)-217(cells)-215.2(\(Fig.)-220.9(1B\),)-215.1(indicating)-221.2(that)-213.2(both)-217.6(recEMab-134)]TJ
T*
[(and)-522.8(134-mG)]TJ
7.3823 0 0 6.5614 365.2723 515.2817 Tm
(2a)Tj
9.843 0 0 9.843 372.3022 516.9258 Tm
[(-f)-520.5(detect)-528.7(endogenous)-520(dEGFR-expressed)-529.4(on)]TJ
-6.0189 -1.1174 TD
[(SNP)-286.1(cells.)]TJ
/F10 1 Tf
8.9663 0 0 8.9663 313.0582 484.0439 Tm
[(Determination)-337.6(of)-336.7(binding)-332.2(afnity)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 467.603 Tm
[(A)-318.7(kinetic)-315.7(analysis)-316.3(of)-313.2(the)-315.6(interactions)-322.3(of)-313.2(134-mG)]TJ
7.3823 0 0 6.5614 518.0031 465.9589 Tm
(2a)Tj
9.843 0 0 9.843 524.9762 467.603 Tm
[(-f)-318.9(with)]TJ
-21.5298 -1.1174 TD
[(SNP)-343.7(cells)-347.7(was)-345(performed)-347.4(via)-344.4(ow)-338.1(cytometry.)-351.2(As)-342.7(shown)-340.2(in)]TJ
0 -1.1116 TD
[(Figure)-203.5(1C,)-196.7(the)]TJ
/F8 1 Tf
5.8576 0 TD
(K)Tj
/F4 1 Tf
7.3823 0 0 6.5614 377.2912 444.0188 Tm
(D)Tj
9.843 0 0 9.843 384.5479 445.6629 Tm
[(for)-193.3(the)-200.4(interaction)-200.4(of)-198(134-mG)]TJ
7.3823 0 0 6.5614 499.2377 444.0188 Tm
(2a)Tj
9.843 0 0 9.843 506.2676 445.6629 Tm
[(-f)-198(with)-195.6(SNP)]TJ
-19.6291 -1.1116 TD
[(cells)-411.1(was)-408.3(1.5)]TJ
/F9 1 Tf
5.6272 0 TD
()Tj
/F4 1 Tf
.72 0 TD
(10)Tj
/F9 1 Tf
7.3823 0 0 6.5614 385.3416 439.0865 Tm
(-)Tj
/F4 1 Tf
.5529 0 TD
(9)Tj
9.843 0 0 9.843 397.1337 434.7211 Tm
[(M,)-408.6(suggesting)-403.7(that)-409(134-mG)]TJ
7.3823 0 0 6.5614 510.1227 433.0771 Tm
(2a)Tj
9.843 0 0 9.843 517.1526 434.7211 Tm
[(-f)-405.3(shows)]TJ
-20.7349 -1.1174 TD
[(high)-281(afnity)-285.4(for)-279.7(SNP)-280.3(cells.)]TJ
/F10 1 Tf
8.9663 0 0 8.9663 313.0582 401.7826 Tm
[(Immunocytochemical)-338.4(analysis)-334.5(against)-333.8(canine)]TJ
0 -1.2203 TD
[(mammary)-337.8(gland)-330.8(tumor)-338.6(cell)-333(line,)-335.1(SNP)-333.7(cells)]TJ
T*
[(using)-338.5(recEMab-134)-333.3(and)-335.7(134-mG)]TJ
6.7246 0 0 5.9768 444.0755 378.3684 Tm
(2a)Tj
8.9663 0 0 8.9663 451.5589 379.8991 Tm
(-f)Tj
/F4 1 Tf
9.843 0 0 9.843 323.0361 363.4582 Tm
.0116 Tc
[(RecEMab-)4.8(13)4.7(4)-415.7(a)-2.2(n)4.7(d)-415.7(13)4.7(4-mG)]TJ
7.3823 0 0 6.5614 437.7826 361.8141 Tm
.0145 Tc
(2a)Tj
9.843 0 0 9.843 445.0392 363.4582 Tm
.4273 Tc
[(-)420.5(f)0(w)413.8(e)419.3(r)414.7(e)-1.2(a)419.3(p)414.7(p)420.4(l)414.6(i)420.3(e)413.5(dt)420.3(oi)414.6(m)419.2(m)413.5(u)420.4(-)]TJ
-13.4086 -1.1116 TD
.0118 Tc
[(nocyt)4.8(ochemi)4.8(str)5(y)-484.6(in)-484.6(SNP)-490.2(c)3.8(el)4.8(ls)5.1(.)-487(A)-1.7(s)-484.4(s)-.6(h)4.9(o)-.8(wn)-490.4(i)4.8(n)-484.6(Fig)4.9(u)-.8(r)5(e)-485.8(1D,)]TJ
0 -1.1174 TD
.0124 Tc
[(recEMab)5.5(-)-.2(1)5.5(3)-.2(4)-380.3(a)4.4(nd)-380.4(13)5.5(4-)5.6(mG)]TJ
7.3823 0 0 6.5614 423.8928 339.8739 Tm
.0145 Tc
(2a)Tj
9.843 0 0 9.843 431.0928 341.518 Tm
.0123 Tc
[(-f,)-377(bu)5.4(t)-380.6(n)-.3(o)5.4(t)-380.6(buf)5.5(fer)-380.4(c)-1.5(o)5.4(n)-.3(tr)5.5(ol)5.3(,)-382.8(v)-.3(i)5.3(-)]TJ
-11.9917 -1.1116 TD
.0092 Tc
[(sualiz)-4.6(ed)-210.8(dEG)-4.3(F)2.5(R)-211.8(i)-3.5(n)-210.8(S)2.5(N)-4.3(P)-210.6(ce)-4.6(lls,)-207.3(sugge)-4.6(s)-3.2(ting)-210.8(tha)-4.6(t)-210.9(re)-4.6(cE)-4.6(Ma)-4.6(b-134)]TJ
T*
.0121 Tc
[(and)-559.2(1)5.2(34)5.2(-mG)]TJ
7.3823 0 0 6.5614 366.5763 317.9905 Tm
.0145 Tc
(2a)Tj
9.843 0 0 9.843 373.7763 319.6346 Tm
.0118 Tc
[(-f)-559.5(reco)4.9(gn)4.9(ize)-566.4(e)3.8(nd)4.9(og)4.9(en)4.9(ous)-559.3(d)4.9(E)-2(GFR)-560.5(i)-.9(n)-559.5(S)-.6(NP)]TJ
-6.1686 -1.1174 TD
.0134 Tc
[(cel)6.4(ls)6.7(.)]TJ
/F10 1 Tf
8.9663 0 0 8.9663 313.0582 286.696 Tm
0 Tc
(134-mG)Tj
6.7246 0 0 5.9768 345.3731 285.222 Tm
(2a)Tj
8.9663 0 0 8.9663 352.8566 286.696 Tm
[(-f-mediated)-335.5(ADCC)-335.1(and)-329.4(CDC)-337.2(in)-330.2(SNP)-340.1(cells)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 270.3117 Tm
[(We)-547(next)-541.4(investigated)-546.9(whether)-546.7(134-mG)]TJ
7.3823 0 0 6.5614 487.5589 268.6677 Tm
(2a)Tj
9.843 0 0 9.843 494.5321 270.3117 Tm
[(-f)-543.5(has)-545.7(ADCC)]TJ
-18.4368 -1.1174 TD
[(again)-8(st)-526.3(SNP)-533.7(cells.)-535.5(As)-527(shown)-536.1(in)-526.5(Figure)-537.6(2A,)-530.6(134-mG)]TJ
7.3823 0 0 6.5614 538.6392 257.6693 Tm
(2a)Tj
9.843 0 0 9.843 545.6125 259.3133 Tm
(-f)Tj
-23.6263 -1.1116 TD
[(showed)-267.6(ADCC)-267.4(\(46.8%)-263.4(cytotoxicity\))-264.9(against)-269.3(SNP)-263(cells)-267.1(more)]TJ
T*
[(potent)-192.3(than)-184.2(mouse)-186.3(IgG)]TJ
7.3823 0 0 6.5614 401.329 235.7858 Tm
(2a)Tj
9.843 0 0 9.843 410.1731 237.4299 Tm
[(\(12.9%)-182.8(cytotoxicity\))-190.1(and)-188.8(PBS)-182.6(\(7.8%)]TJ
-9.8664 -1.1174 TD
[(cytotoxicit)-9.3(y\).)]TJ
1.0137 -1.1116 TD
[(We)-662.2(then)-668.1(investigated)-662.1(whether)-667.6(134-mG)]TJ
7.3823 0 0 6.5614 492.2644 213.8456 Tm
(2a)Tj
9.843 0 0 9.843 499.2943 215.4897 Tm
[(-f)-658.7(has)-666.6(CDC)]TJ
-18.9206 -1.1116 TD
[(again)-8(st)-324.7(SNP)-332.2(cells.)-328.1(As)-331.2(shown)-328.7(in)-330.6(Figure)-330.2(2B,)-323.5(134-mG)]TJ
7.3823 0 0 6.5614 522.1416 202.9039 Tm
(2a)Tj
9.843 0 0 9.843 529.1715 204.548 Tm
[(-f)-330.4(eli-)]TJ
-21.956 -1.1174 TD
[(cited)-347.9(a)-347.8(higher)-347.7(degree)-344.1(of)-347.7(CDC)-346.1(\(59.5%)-344(cytotoxicity\))-351.3(in)-342.2(SNP)]TJ
0 -1.1116 TD
[(cells)-434.1(compared)-434(with)-431.8(that)-426.3(elicited)-433.3(by)-428.4(mouse)-433.9(IgG)]TJ
7.3823 0 0 6.5614 512.2203 180.9638 Tm
(2a)Tj
9.843 0 0 9.843 523.4455 182.6078 Tm
(\(27.3%)Tj
-21.3743 -1.1116 TD
[(cytotoxicit)-9.3(y\))-434.1(and)-436.4(PBS)-441.8(\(18.5%)-436.2(cytotoxicity\).)-441.2(These)-440.8(results)]TJ
0 -1.1174 TD
[(demonstrated)-674.6(that)-668.2(134-mG)]TJ
7.3823 0 0 6.5614 427.0676 159.0803 Tm
(2a)Tj
9.843 0 0 9.843 434.0975 160.6676 Tm
[(-f)-664.5(exerts)-671.2(ADCC)-670.6(and)-666.8(CDC)]TJ
-12.297 -1.1116 TD
[(again)-8(st)-278.6(dEGFR-expressing)-287.5(SNP)-280.3(cells.)]TJ
/F10 1 Tf
8.9663 0 0 8.9663 313.0582 127.7858 Tm
[(Antitumor)-341.4(activities)-334.2(of)-330.4(134-mG)]TJ
6.7246 0 0 5.9768 434.381 126.3118 Tm
(2a)Tj
8.9663 0 0 8.9663 441.8077 127.7858 Tm
[(-f)-334.4(in)-336.5(the)-334.8(mouse)]TJ
-14.3593 -1.2203 TD
[(xenografts)-333.8(of)-336.7(SNP)-333.7(cells)]TJ
/F4 1 Tf
9.843 0 0 9.843 323.0361 100.4031 Tm
[(In)-411.1(the)-407.8(SNP)-407(xenograft)-415.5(models,)-409.8(134-mG)]TJ
7.3823 0 0 6.5614 483.0235 98.759 Tm
(2a)Tj
9.843 0 0 9.843 489.9967 100.4031 Tm
[(-f)-411.1(\(100)]TJ
/F11 1 Tf
3.0757 0 TD
(l)Tj
/F4 1 Tf
.5529 0 TD
[(g\))-411.1(and)]TJ
-21.6047 -1.1116 TD
[(control)-354.7(mouse)-353.3(IgG)-355.5(\(100)]TJ
/F11 1 Tf
10.068 0 TD
(l)Tj
/F4 1 Tf
.5529 0 TD
[(g\))-353.5(were)-353.2(injected)-358.3(intraperitoneally)]TJ
-10.6209 -1.1174 TD
[(into)-183.2(mice)-186.6(on)-180.8(days)-183.9(7,)-183.1(11,)-184.2(and)-183(18)-180.8(following)-183.9(the)-183.1(injection)-185.6(of)-186.5(SNP)]TJ
0 -1.1116 TD
[(cells.)-379.9(The)-374.3(tumor)-381.2(volume)-372(was)-379.5(measured)-376.2(on)-376.6(days)-379.8(7,)-373.2(11,)-374.3(14,)]TJ
T*
[(18,)-477.9(21,)-477.9(and)-482.5(25)-474.5(after)-480.2(the)-482.6(injection.)-482.7(The)-478(administration)-480.2(of)]TJ
/F6 1 Tf
8.9663 0 0 8.9663 62.022 749.6502 Tm
(144)Tj
46.897 0 TD
[(TANAKA)-335.9(ET)-338.7(AL.)]TJ
ET
endstream
endobj
46 0 obj
<>stream
/GS1 gs
BT
/F4 1 Tf
9.843 0 0 9.843 59.7543 100.4598 Tm
0 0 0 rg
0 Tc
0 Tw
(134-mG)Tj
7.3823 0 0 6.5614 92.5795 98.8157 Tm
(2a)Tj
9.843 0 0 9.843 99.6094 100.4598 Tm
[(-f)-342(resulted)-345.2(in)-347.9(a)-342.1(signicant)-347.4(reduction)-346.5(in)-342.1(tumor)-340.9(de-)]TJ
-4.0491 -1.1116 TD
[(velopment)-549.4(on)-537.9(days)-546.8(18)-537.8(\()]TJ
/F8 1 Tf
10.7419 0 TD
(p)Tj
/F9 1 Tf
.6681 0 TD
(<)Tj
/F4 1 Tf
.72 0 TD
[(0.05\),)-541.1(21)-537.9(\()]TJ
/F8 1 Tf
4.913 0 TD
(p)Tj
/F9 1 Tf
.6681 0 TD
(<)Tj
/F4 1 Tf
.72 0 TD
[(0.05\),)-541.1(and)-540.1(25)]TJ
-18.4311 -1.1174 TD
(\()Tj
/F8 1 Tf
.5011 0 TD
(p)Tj
/F9 1 Tf
.6681 0 TD
(<)Tj
/F4 1 Tf
.72 0 TD
[(0.01\))-497.4(compared)-503.1(with)-500.9(that)-501.2(of)-503.3(the)-499.9(control)-504.4(mouse)-497.3(IgG)]TJ
-1.8892 -1.1116 TD
[(\(Fig.)-411(3A\).)-405(The)-408.8(administration)-411.1(of)-411.1(134-mG)]TJ
7.3823 0 0 6.5614 229.8897 65.9338 Tm
(2a)Tj
9.843 0 0 9.843 236.9196 67.5779 Tm
[(-f)-411.1(resulted)-408.6(in)-405.5(a)]TJ
-17.9991 -1.1116 TD
[(31%)-349.9(reduction)-346.5(of)-347.7(tumor)-346.7(volume)-349(compared)-347.6(with)-345.4(that)-351.4(of)-342(the)]TJ
25.5097 4.4523 TD
[(control)-239.5(mouse)-238.1(IgG)-240.3(on)-238.3(day)-240.6(25)-238.3(postinjection.)-239.4(Furthermore,)-243.6(the)]TJ
0 -1.1116 TD
[(tumor)-427.3(weight)-423.6(of)-428.4(the)-425.1(134-mG)]TJ
7.3823 0 0 6.5614 431.1495 87.874 Tm
(2a)Tj
9.843 0 0 9.843 438.1227 89.5181 Tm
[(-f-treated)-429.3(mice)-428.6(was)-425.6(signi-)]TJ
-12.9305 -1.1174 TD
[(cantly)-331.7(lower)-335.9(than)-334(that)-334.1(of)-330.5(IgG-treated)-339.2(mice)-330.6(\(21%)-333.7(reduction;)]TJ
/F8 1 Tf
0 -1.1116 TD
(p)Tj
/F9 1 Tf
.6681 0 TD
(<)Tj
/F4 1 Tf
.7142 0 TD
[(0.05,)-280.9(Fig.)-277.4(3B\).)-278.5(Total)-278.8(body)-275.1(weights)-280.5(of)-278.6(the)-275.3(two)-280.8(groups)-277.1(did)]TJ
-1.3823 -1.1116 TD
[(not)-279.9(change)-280.8(signicantly)-286.4(\(Fig.)-278.5(3C\).)]TJ
ET
q
336.132 0 0 508.479 136.8 223.767 cm
/Im2 Do
Q
BT
/F5 1 Tf
9.845 0 0 9.845 59.7543 206.9858 Tm
[(FIG.)-336.7(1.)]TJ
/F4 1 Tf
4.1174 0 TD
[(Flow)-333(cytometry)-335.2(and)-332.4(immunocytochemistry)-337.3(using)-333.2(recEMab-134)-338.9(and)-332.4(134-mG)]TJ
7.3837 0 0 6.5625 409.3227 205.3417 Tm
(2a)Tj
9.845 0 0 9.845 416.3526 206.9858 Tm
(-f.)Tj
/F5 1 Tf
1.2496 0 TD
[(\(A,)-334.4(B\))]TJ
/F4 1 Tf
2.9656 0 TD
[(SNP)-337.4(cells)-335.7(were)-335.4(treated)]TJ
-40.4365 -1.0135 TD
[(with)-321.9(recEMab-134)]TJ
/F5 1 Tf
7.9353 0 TD
(\(A\))Tj
/F4 1 Tf
1.7045 0 TD
[(and)-320.9(134-mG)]TJ
7.3837 0 0 6.5625 204.8881 195.3637 Tm
(2a)Tj
9.845 0 0 9.845 211.8614 197.0078 Tm
(-f)Tj
/F5 1 Tf
.9905 0 TD
(\(B\))Tj
/F4 1 Tf
1.6527 0 TD
[(or)-318.7(buffer)-323.8(control)]TJ
/F5 1 Tf
7.083 0 TD
[(\(A,)-322.9(B\))]TJ
/F4 1 Tf
2.6201 0 TD
[(,)-320.1(followed)-321.2(by)-324.5(Alexa)-319.3(Fluor)-321.7(488-conjugated)-322.9(anti-mouse)]TJ
-27.7965 -1.0135 TD
(IgG.)Tj
/F5 1 Tf
2.0443 0 TD
(\(C\))Tj
/F4 1 Tf
1.6297 0 TD
[(Determination)-249.4(of)-238.1(the)-246.2(binding)-243.4(afnity)-244.4(of)-238.1(134-mG)]TJ
7.3837 0 0 6.5625 287.3196 185.3858 Tm
(2a)Tj
9.845 0 0 9.845 294.3495 187.0298 Tm
[(-f)-243.8(for)-239.1(SNP)-245.3(cells)-243.6(using)-241.1(ow)-245.5(cytometry.)-246.5(SNP)-239.5(cells)-243.6(were)-249(suspended)]TJ
-23.8289 -1.0077 TD
[(in)-514.7(100)]TJ
/F12 1 Tf
2.9599 0 TD
(m)Tj
/F4 1 Tf
.5528 0 TD
[(L)-508.9(o)0(f)-508.7(serially)-517.4(diluted)-509.6(134-mG)]TJ
7.3837 0 0 6.5625 218.0976 175.4645 Tm
(2a)Tj
9.845 0 0 9.845 225.1275 177.1086 Tm
[(-f,)-506.4(followed)-511.3(by)-514.5(the)-511.1(addition)-510.6(of)-508.7(Alexa)-515(Fluor)-511.7(488-conjugated)-512.9(anti-mouse)-508.8(IgG.)]TJ
-16.7977 -1.0135 TD
[(Fluorescence)-461(data)-449.9(were)-456.3(collected)-457.5(using)-448.4(the)-453.5(EC800)-456.3(Cell)-449.9(Analyzer.)]TJ
/F5 1 Tf
28.4011 0 TD
(\(D\))Tj
/F4 1 Tf
1.837 0 TD
[(SNP)-452.6(cells)-450.9(were)-456.3(incubated)-456.2(with)-448.5(buffer)-456.3(control,)]TJ
-30.2381 -1.0135 TD
(10)Tj
/F12 1 Tf
1.169 0 TD
(m)Tj
/F4 1 Tf
.5528 0 TD
[(g/mL)-328.9(of)-336(recEMab-134,)-336.5(or)-330.2(10)]TJ
/F12 1 Tf
12.0987 0 TD
(m)Tj
/F4 1 Tf
.5471 0 TD
[(g/mL)-334.7(of)-330.2(134-mG)]TJ
7.3837 0 0 6.5625 270.1983 155.5086 Tm
(2a)Tj
9.845 0 0 9.845 277.2283 157.1527 Tm
[(-f)-330.2(for)-337(1)-329.2(hour)-332.2(at)-331.6(room)-333.4(temperature.)-337.2(The)-333.5(cells)-335.7(were)-329.6(further)-336.5(incubated)]TJ
-22.0898 -1.0135 TD
[(with)-281.5(Alexa)-284.7(Fluor)-281.4(488-conjugated)-282.6(anti-mouse)-284.2(IgG)-280.2(and)-286.3(DAPI)-281.8(for)-279.4(45)-278.4(minutes)-285.9(at)-279.7(room)-281.5(temperature.)-285.4(Scale)-284.9(bars,)-279(20)]TJ
/F12 1 Tf
45.3543 0 TD
(m)Tj
/F4 1 Tf
.5528 0 TD
[(m.)-281.9(DAPI,)]TJ
-45.9071 -1.0077 TD
(4)Tj
/F9 1 Tf
.501 0 TD
()Tj
/F4 1 Tf
.2534 0 TD
[(,6-diamidino-2-phenylindole;)-335.4(recEMab-134,)-336.5(recombinant)-338.4(EMab-134.)]TJ
/F6 1 Tf
8.9663 0 0 8.9663 59.7543 749.7069 Tm
[(ANTITUMOR)-340.6(ACTIVITIES)-337.4(BY)-335.8(DEFUCOSYLATED)-335.5(ANTI-EGFR)-336.2(MAB)-21852.9(145)]TJ
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