研究日記(ラボのトピックス)

  • 2019/6/23(日)

    第19回日本蛋白質科学会年会にて、3つの演題を発表します。

    会議名 : 第19回日本蛋白質科学会年会
    演題 : 抗MAP タグ抗体の結晶構造とMAP タグシステムの新たな応用法
    発表者 : 有森貴夫、若狹彩美、金子美華、加藤幸成、高木 淳一
    期日 : 2019年6月24日(月)
    会場 : 神戸国際会議場,神戸国際展示場、神戸市

    会議名 : 第19回日本蛋白質科学会年会
    演題 : 電子顕微鏡単粒子解析に向けた抗体断片の結合による標的タンパク質のサイズと形状の最適化
    発表者 : 三好賢一、田村梨沙子、高貫一徳、廣瀬未果、大井里香、金子美華、加藤幸成、岩崎憲治、禾晃和
    期日 : 2019年6月25日(火)
    会場 : 神戸国際会議場,神戸国際展示場、神戸市

    会議名 : 第19回日本蛋白質科学会年会
    演題 : モノクローナル抗体の詳細と提供情報のデータベース
    発表者 : 鈴木博文、金子美華、加藤幸成、由良 敬
    期日 : 2019年6月25日(火)
    会場 : 神戸国際会議場,神戸国際展示場、神戸市

  • 2019/6/23(日)

    自分の論文からの新しい引用

  • 2019/6/20(木)

    PubMed掲載。

    Jangphattananont N, Sato H, Imamura R, Sakai K, Terakado Y, Murakami K, Barker N, Oshima H, Oshima M, Takagi J, Kato Y, Yano S, Matsumoto K.
    Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach
    Int. J. Mol. Sci.,20(12), 2955; https://doi.org/10.3390/ijms20122955, 2019 (PDF)

    Abstract
    Hepatocyte growth factor (HGF) is secreted as an inactive single-chain HGF (scHGF); however, only proteolytically processed two-chain HGF (tcHGF) can activate the MET receptor. We investigated the localization of tcHGF and activated/phosphorylated MET (pMET) using a tcHGF-specific antibody. In day 16.5 mouse embryos, total HGF (scHGF + tcHGF) was mainly localized in smooth muscle cells close to, but separate from, MET-positive epithelial cells in endodermal organs, including the stomach. In the adult stomach, total HGF was localized in smooth muscle cells, and tcHGF was mainly localized in the glandular base region. Immunostaining for pMET and Lgr5-driven green fluorescent protein (GFP) indicated that pMET localization overlapped with Lgr5+ gastric stem cells. HGF promoted organoid formation similar to EGF, indicating the potential for HGF to promote the survival and growth of gastric stem cells. pMET and tcHGF localizations changed during regeneration following gastric injury. These results indicate that MET is constantly activated in gastric stem cells and that the localization of pMET differs from the primary localization of precursor HGF but has a close relationship to tcHGF. Our results suggest the importance of the microenvironmental generation of tcHGF in the regulation of development, regeneration, and stem cell behavior.

    KEYWORDS:
    HGF; MET receptor; regeneration; smooth muscle cell; stem cell
    PMID: 31212972
    DOI: 10.3390/ijms20122955

  • 2019/6/19(水)

    PMab-247のIHC論文がMABにアクセプトされました。

    Junko Takei, Yoshikazu Furusawa, Shinji Yamada, Takuro Nakamura, Yusuke Sayama, Masato Sano, Satoru Konnai, Atsushi Kobayashi, Hiroyuki Harada, Mika K. Kaneko, Yukinari Kato*.
    (*corresponding author)

    PMab-247 Detects Bear Podoplanin in Immunohistochemical Analysis.
    Monoclon. Antib. Immunodiagn. Immunother.in press

  • 2019/6/18(火)

    自分の論文からの新しい引用

  • 2019/6/17(月)

    共著論文がInt. J. Mol. Sci.に掲載されました。

    Nawaphat Jangphattananont, Hiroki Sato, Ryu Imamura, Katsuya Sakai, Yumi Terakado, Kazuhiro Murakami, Nick Barker, Hiroko Oshima, Masanobu Oshima, Junichi Takagi, Yukinari Kato, Seiji Yano, Kunio Matsumoto
    Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach
    Int. J. Mol. Sci., 20(12), 2955; https://doi.org/10.3390/ijms20122955, 2019 (PDF)

  • 2019/6/15(土)

    H2Mab-41の論文がPubMedに掲載。

    Kato Y*, Ohishi T, Yamada S, Itai S, Takei J, Sano M, Nakamura T, Harada H, Kawada M, Kaneko MK.
    (*corresponding author)

    Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody H2Mab-41 Exerts Antitumor Activity in a Mouse Xenograft Model of Colon Cancer.
    Monoclon. Antib. Immunodiagn. Immunother., https://doi.org/10.1089/mab.2019.0017, 2019 (PDF)

  • 2019/6/15(土)

    MABのonlineに掲載。

    Yukinari Kato*, Tomokazu Ohishi, Shinji Yamada, Shunsuke Itai, Junko Takei, Masato Sano, Takuro Nakamura, Hiroyuki Harada, Manabu Kawada, Mika K. Kaneko.
    (*corresponding author)

    Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody H2Mab-41 Exerts Antitumor Activity in a Mouse Xenograft Model of Colon Cancer.
    Monoclon. Antib. Immunodiagn. Immunother., https://doi.org/10.1089/mab.2019.0017, 2019 (PDF)

  • 2019/6/13(木)

    6報の論文がMAB6月号に掲載されました。

    Kato Y*, Furusawa Y, Itai S, Takei J, Nakamura T, Sano M, Harada H, Yamada S, Kaneko MK.
    (*corresponding author)

    Establishment of an anti-cetacean podoplanin monoclonal antibody PMab-237 for immunohistochemical analysis
    Monoclon. Antib. Immunodiagn. Immunother., 38(3), 108–113, https://doi.org/10.1089/mab.2019.0013, 2019 (PDF)

    Yamada S, Itai S, Nakamura T, Takei J, Sano M, Konnai S, Kobayashi A, Nakagun S, Kobayashi Y, Kaneko MK, Kato Y.*
    (*corresponding author)

    Immunohistochemical analysis of the harbor porpoise using anti-podoplanin antibody PMab-237
    Monoclon. Antib. Immunodiagn. Immunother., 38(3), 104–107, https://doi.org/10.1089/mab.2019.0014, 2019 (PDF)

    Sano M, Kaneko MK, Kato Y.*.
    (*corresponding author)

    Epitope Mapping of Anti-Human DGKgamma Monoclonal Antibody DgMab-6
    Monoclon. Antib. Immunodiagn. Immunother., 38(3), 120–123, https://doi.org/10.1089/mab.2019.0011, 2019 (PDF)

    Kato Y*, Itai S, Yamada S, Suzuki H, Kaneko MK. Kaneko
    (*corresponding author)

    Epitope Mapping of Anti-Diacylglycerol Kinase Zeta Monoclonal Antibody for the Detection of T cells by Immunohistochemical Analyses
    Monoclon. Antib. Immunodiagn. Immunother., 38(3), 124–128, doi: 10.1089/mab.2019.0005., 2019 (PDF)

    Takei J, Itai S, Furusawa Y, Yamada S, Nakamura T, Sano M, Harada H, Fukui M, Kaneko MK, Kato Y*
    (*corresponding author)

    Epitope mapping of anti-tiger podoplanin monoclonal antibody PMab-231
    Monoclon. Antib. Immunodiagn. Immunother., 38(3), 129–132, doi: 10.1089/mab.2019.0012., 2019 (PDF)

    Kaneko MK, Yamada S, Itai S, Chang YW, Nakamura T, Yanaka M, Harada H, Suzuki H, Kato Y.*
    (*corresponding author)

    Elucidation of the TMab-6 Monoclonal Antibody Epitope Against Telomerase Reverse Transcriptase
    Monoclon. Antib. Immunodiagn. Immunother., 38(3), 101–103, https://doi.org/10.1089/mab.2018.0007, 2019 (PDF)

  • 2019/6/13(木)

    共著論文1報 がnternational Journal of Molecular Sciencesにアクセプトされました。

  • 2019/6/12(水)

    第23回日本がん分子標的治療学会学術集会にて、2つの演題を発表します。

    会議名 : 第23回日本がん分子標的治療学会学術集会
    演題 : 悪性胸膜中皮腫に対するがん特異的抗podoplanin抗体chLpMab-2のin vitroにおける抗腫瘍効果の検討
    発表者 : 和泉俊尋、阿部真治、後東久嗣、加藤幸成、西岡安彦
    期日 : 2019年6月13(木)
    会場 : 大阪国際交流センター、大阪市

    会議名 : 第23回日本がん分子標的治療学会学術集会
    演題 : Universal" CTC-chipと抗podoplanin抗体NZ-1.2を用いた悪性胸膜中皮腫における循環腫瘍細胞(CTCs)の検出
    発表者 : 米田和恵、加藤幸成、田中文啓
    期日 : 2019年6月13(木)
    会場 : 大阪国際交流センター、大阪市

  • 2019/6/11(火)

    自分の論文からの新しい引用

  • 2019/6/9(日)

    自分の論文からの新しい引用

  • 2019/6/6(木)

    PMab-237(クジラポドプラニンに対する抗体)の2報がPubMedに掲載されました。

    Kato Y*, Furusawa Y, Itai S, Takei J, Nakamura T, Sano M, Harada H, Yamada S, Kaneko MK. Kaneko
    (*corresponding author)

    Establishment of an anti-cetacean podoplanin monoclonal antibody PMab-237 for immunohistochemical analysis
    Monoclon. Antib. Immunodiagn. Immunother., Published Online:4 Jun 2019https://doi.org/10.1089/mab.2019.0013, 2019 (PDF)

    Yamada S, Itai S, Nakamura T, Takei J, Sano M, Konnai S, Kobayashi A, Nakagun S, Kobayashi Y, Kaneko MK, Kato Y.*
    (*corresponding author)

    Immunohistochemical analysis of the harbor porpoise using anti-podoplanin antibody PMab-237
    Monoclon. Antib. Immunodiagn. Immunother., Published Online:4 Jun 2019https://doi.org/10.1089/mab.2019.0014, 2019 (PDF)

  • 2019/6/6(木)

    自分の論文からの新しい引用

  • 2019/6/4(火)

    PMab-237(クジラポドプラニンに対する抗体)の2報がMABに掲載されました。

    Yukinari Kato*, Yoshikazu Furusawa, Shunsuke Itai, Junko Takei, Takuro Nakamura, Masato Sano, Hiroyuki Harada, Shinji Yamada, Mika K. Kaneko
    (*corresponding author)

    Establishment of an anti-cetacean podoplanin monoclonal antibody PMab-237 for immunohistochemical analysis
    Monoclon. Antib. Immunodiagn. Immunother., Published Online:4 Jun 2019https://doi.org/10.1089/mab.2019.0013, 2019 (PDF)

    Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Junko Takei, Masato Sano, Satoru Konnai, Atsushi Kobayashi, Shotaro Nakagun, Yoshiyasu Kobayashi, Mika K. Kaneko, Yukinari Kato*
    (*corresponding author)

    Immunohistochemical analysis of the harbor porpoise using anti-podoplanin antibody PMab-237
    Monoclon. Antib. Immunodiagn. Immunother., Published Online:4 Jun 2019https://doi.org/10.1089/mab.2019.0014, 2019 (PDF)



  • 2019/6/4(火)

    Monoclon Antib Immunodiagn Immunother. 2019 May 31. doi: 10.1089/mab.2019.0011. [Epub ahead of print]

    Epitope Mapping of Antihuman Diacylglycerol Kinase Gamma Monoclonal Antibody DgMab-6.

    Sano M, Kaneko MK, Kato Y.

    Abstract

    Diacylglycerol kinase (DGK) converts diacylglycerol (DG) into phosphatidic acid (PA). Both DG and PA serve as lipidic second messengers; therefore, DGK plays a critical role in regulating the balance of two signaling pathways mediated by DG and PA in cellular functions. DGK gamma (DGKγ), 1 of the 10 mammalian DGK isozymes, has been reported to be involved in membrane traffic, mast cell function, and leukemic cell differentiation. We previously developed a novel anti-DGKγ monoclonal antibody (mAb), DgMab-6, which is very useful in immunocytochemistry of human cultured cells. In this study, we characterized the binding epitope of DgMab-6 using Western blotting, and Glu12 is important for facilitating the DgMab-6 binding to the DGKγ protein. These results might lead to further development for sensitive and specific mAbs against DGKγ.

    KEYWORDS:

    DGKγ; DgMab-6; diacylglycerol kinase; monoclonal antibody

    PMID: 31149888
    DOI: 10.1089/mab.2019.0011

  • 2019/6/4(火)

    自分の論文からの新しい引用

  • 2019/6/3(月)

    ファルマシア6月号に掲載されました。

    研究室から
    革新的がん特異的抗体の開発の現状と展望
    アカデミア発の抗体医薬開発を目指して

  • 2019/6/1(土)

    DgMab-6のエピトープ解析論文がMABに掲載されました。

    Masato Sano, Mika K. Kaneko, Yukinari Kato*.
    (*corresponding author)

    Epitope Mapping of Anti-Human DGKgamma Monoclonal Antibody DgMab-6
    Monoclon. Antib. Immunodiagn. Immunother., https://doi.org/10.1089/mab.2019.0011, 2019 (PDF)

  • 2019/6/1(土)

    自分の論文からの新しい引用

  • 2019/5/28(火)

    20種類のanti-EGFR mAbについて、遺伝子クローニングが終了。
    抗体バンクに登録済み。

  • 2019/5/25(土)

    自分の論文からの新しい引用

  • 2019/5/22(水)

    PubMed掲載:2報

  • 2019/5/22(水)

    PMab-231のエピトープ解析の論文とDGKzeta関連の論文がonlineに掲載されました。

    Kato Y*, Itai S, Yamada S, Suzuki H, Kaneko MK. Kaneko
    (*corresponding author)

    Epitope Mapping of Anti-Diacylglycerol Kinase Zeta Monoclonal Antibody for the Detection of T cells by Immunohistochemical Analyses
    Monoclon. Antib. Immunodiagn. Immunother., 2019 May 21. doi: 10.1089/mab.2019.0005. [Epub ahead of print](PDF)

    Takei J, Itai S, Furusawa Y, Yamada S, Nakamura T, Sano M, Harada H, Fukui M, Kaneko MK, Kato Y*
    (*corresponding author)

    Epitope mapping of anti-tiger podoplanin monoclonal antibody PMab-231
    Monoclon. Antib. Immunodiagn. Immunother., 2019 May 21. doi: 10.1089/mab.2019.0012. [Epub ahead of print](PDF)

  • 2019/5/21(火)

    自分の論文からの新しい引用

  • 2019/5/20(月)

    金沢大学など、活性型HGFのみ阻害する環状ペプチドを発見

    金沢大学、東京大学、理化学研究所の共同研究グループは、癌細胞の転移や抗癌剤耐性を促進する活性型肝細胞増殖因子(HGF)と特異的に結合して機能を阻害する環状ペプチド「HGF-inhibitory Peptide-8(HiP-8)」を発見したと発表した。ポジトロン断層撮影(PET)診断用プローブとしても活用でき、癌進行のモニタリングや治療薬への応用に期待がかかる。成果はNature Chemical Biologyに17日掲載された。

  • 2019/5/20(月)

    ”Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor”の論文がPubMedに掲載。

    Sakai K, Passioura T, Sato H, Ito K, Furuhashi H, Umitsu M, Takagi J, Kato Y, Mukai H, Warashina S, Zouda M, Watanabe Y, Yano S, Shibata M, Suga H, Matsumoto K.
    Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor.
    Nat Chem Biol., 15(6):598-606. doi: 10.1038/s41589-019-0285-7, 2019

    Abstract
    Activation of hepatocyte growth factor (HGF) by proteolytic processing is triggered in cancer microenvironments, and subsequent signaling through the MET receptor is involved in cancer progression. However, the structure of HGF remains elusive, and few small/medium-sized molecules can modulate HGF. Here, we identified HiP-8, a macrocyclic peptide consisting of 12 amino acids, which selectively recognizes active HGF. Biochemical analysis and real-time single-molecule imaging by high-speed atomic force microscopy demonstrated that HiP-8 restricted the dynamic domains of HGF into static closed conformations, resulting in allosteric inhibition. Positron emission tomography using HiP-8 as a radiotracer enabled noninvasive visualization and simultaneous inhibition of HGF–MET activation status in tumors in a mouse model. Our results illustrate the conformational change in proteolytic activation of HGF and its detection and inhibition by a macrocyclic peptide, which may be useful for diagnosis and treatment of cancers.

  • 2019/5/18(土)

    DaMab-8の論文がPubMed掲載。

    Sano M, Kaneko MK, Suzuki H, Kato Y.*
    (*corresponding author)

    Establishment and Epitope Mapping of Anti-Diacylglycerol Kinase Alpha Monoclonal Antibody DaMab-8 for immunohistochemical analyses
    Monoclon. Antib. Immunodiagn. Immunother., doi: 10.1089/mab.2019.0004.(PDF)

  • 2019/5/18(土)

    H2Mab-41の論文がアクセプトされました。2019年の25報目です。

    Yukinari Kato*, Tomokazu Ohishi, Shinji Yamada, Shunsuke Itai, Junko Takei, Masato Sano, Takuro Nakamura, Hiroyuki Harada, Manabu Kawada, Mika K. Kaneko.
    (*corresponding author)
    Anti-Human Epidermal Growth Factor Receptor 2 Monoclonal Antibody H2Mab-41 Exerts Antitumor Activity in a Mouse Xenograft Model of Colon Cancer.
    Monoclon. Antib. Immunodiagn. Immunother., in press

  • 2019/5/18(土)

    DaMab-8の論文がMAB掲載。

    Sano M, Kaneko MK, Suzuki H, Kato Y.*
    (*corresponding author)

    Establishment and Epitope Mapping of Anti-Diacylglycerol Kinase Alpha Monoclonal Antibody DaMab-8 for immunohistochemical analyses
    Monoclon. Antib. Immunodiagn. Immunother., doi: 10.1089/mab.2019.0004.(PDF)

  • 2019/5/18(土)

    自分の論文からの新しい引用

  • 2019/5/17(金)

    ”Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor”の論文がNature Chemical Biologyに掲載。

    Sakai K, Passioura T, Sato H, Ito K, Furuhashi H, Umitsu M, Takagi J, Kato Y, Mukai H, Warashina S, Zouda M, Watanabe Y, Yano S, Shibata M, Suga H, Matsumoto K.
    Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor.
    Nat Chem Biol., 15(6):598-606. doi: 10.1038/s41589-019-0285-7, 2019

    Abstract
    Activation of hepatocyte growth factor (HGF) by proteolytic processing is triggered in cancer microenvironments, and subsequent signaling through the MET receptor is involved in cancer progression. However, the structure of HGF remains elusive, and few small/medium-sized molecules can modulate HGF. Here, we identified HiP-8, a macrocyclic peptide consisting of 12 amino acids, which selectively recognizes active HGF. Biochemical analysis and real-time single-molecule imaging by high-speed atomic force microscopy demonstrated that HiP-8 restricted the dynamic domains of HGF into static closed conformations, resulting in allosteric inhibition. Positron emission tomography using HiP-8 as a radiotracer enabled noninvasive visualization and simultaneous inhibition of HGF–MET activation status in tumors in a mouse model. Our results illustrate the conformational change in proteolytic activation of HGF and its detection and inhibition by a macrocyclic peptide, which may be useful for diagnosis and treatment of cancers.

  • 2019/5/16(木)

    自分の論文からの新しい引用

    Probing Glycoforms of Individual Proteins Using Antibody‐Lectin Sandwich Arrays: Methods and Findings from Studies of Pancreatic Cancer
    BB Haab - Proteomics for Biological Discovery, 2019
    Changes to the glycosylation of a protein can greatly affect the biophysical interactions and intrinsic structural properties of the protein. Because disease‐ associated changes in glycosylation appear in unique forms on specific proteins … Twitter Facebook

    Introducing a New Model of Sweet Taste Receptor, a Class C G-protein Coupled Receptor (C GPCR)
    E Kashani-Amin, A Sakhteman, B Larijani… - Cell biochemistry and …, 2019
    The structure of sweet taste receptor (STR), a heterodimer of class C G-protein coupled receptors comprising T1R2 and T1R3 molecules, is still undetermined. In this study, a new enhanced model of the receptor is introduced based on the most …

  • 2019/5/15(水)

    自分の論文からの新しい引用

    Differential binding of anti-Reelin monoclonal antibodies reveals the characteristics of Reelin protein under various conditions
    K Ishii, T Kohno, M Hattori - Biochemical and Biophysical Research …, 2019
    Reelin is a large secreted protein that is essential for the development and function of the central nervous system. Dimerization and/or oligomerization is required for its biological activity, but the underlying mechanism is not fully understood. There are …

  • 2019/5/13(月)

    BINDSで運営している抗体バンクにおいては、抗体遺伝子クローニングも実施しています。現在、EGFRに対する20種類の抗体の遺伝子クローニングを継続しています。これまでに19種類の抗体遺伝子クローニングが終了し、バンクに登録しました。

  • 2019/5/9(木)

    自分の論文からの新しい引用

    Venous Thromboembolism in Brain Tumors: Risk Factors, Molecular Mechanisms, and Clinical Challenges
    J Riedl, C Ay - Seminars in thrombosis and hemostasis, 2019
    Venous thromboembolism (VTE) is a common complication in patients with primary brain tumors, with up to 20% of patients per year having a VTE event. Clinical risk factors for VTE include glioblastoma subtype, paresis, or surgery. Furthermore …

  • 2019/5/8(水)

    PubMedに掲載されました。

    Biochem Biophys Rep. 2019 Apr 28;18:100644. doi: 10.1016/j.bbrep.2019.100644. eCollection 2019 Jul.

    Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis.

    Furusawa Y1,2, Takei J1, Sayama Y1, Yamada S1, Kaneko MK1, Kato Y1,2.

    Abstract

    Sensitive and specific monoclonal antibodies (mAbs) targeting podoplanin (PDPN) are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues because PDPN is known as a lymphatic endothelial cell maker in pathology. Recently, we established anti-PDPN mAbs against many species, such as human, mouse, rat, rabbit, dog, cat, bovine, pig, horse, goat, tiger, alpaca, and Tasmanian devil. However, anti-bear PDPN (bPDPN) has not been established yet. In this study, we immunized mice with bPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/bPDPN) cells, and screened mAbs against bPDPN using flow cytometry. One of the mAbs, PMab-247 (IgG1, kappa), specifically detected CHO/bPDPN cells by flow cytometry and immunohistochemistry. Our findings suggest the potential usefulness of PMab-247 for the functional analyses of bPDPN.

    PMID: 31061899 PMCID: PMC6488525 DOI: 10.1016/j.bbrep.2019.100644

  • 2019/5/8(水)

    自分の論文からの新しい引用

    [HTML] HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions DC Ranawakage, T Takada, Y Kamachi - Scientific Reports, 2019 The affinity of an antibody for its antigen serves as a critical parameter for antibody evaluation. The evaluation of antibody-antigen affinity is essential for a successful antibody-based assay, particularly immunoprecipitation (IP), due to its strict …

    Role of a Promoter Mutation in TERT in Malignant Transformation of Pleomorphic Xanthoastrocytoma J Hosono, M Nitta, K Masui, T Maruyama, T Komori… - World neurosurgery, 2018 Background Pleomorphic xanthoastrocytoma (PXA) is categorized as grade II, other astrocytic tumors per the 2016 World Health Organization classification. Despite being a relatively benign type of tumor, PXA often has an aggressive clinical course …

    Increasing Bovine leukemia virus (BLV) proviral load is a risk factor for progression of Enzootic bovine leucosis: a prospective study in Japan T Kobayashi, Y Inagaki, N Ohonuki, R Sato… - Preventive Veterinary …, 2019 Bovine leukemia virus (BLV) belongs to the genus Deltaretrovirus in the family Retroviridae, and is etiologically associated with Enzootic Bovine Leukosis (EBL). The majority of BLV-infected cattle remain asymptomatic throughout their productive …

  • 2019/5/6(月)

    90Y-labeled anti-podoplanin antibody NZ-12の論文が、Cancer Scienceの5月号に掲載されました。

    Sudo H, Tsuji AB, Sugyo A, Saga T, Kaneko MK, Kato Y, Higashi T.
    Therapeutic efficacy evaluation of radioimmunotherapy with 90Y-labeled anti-podoplanin antibody NZ-12 for mesothelioma
    Cancer Sci., 110(5):1653-1664, https://doi.org/10.1111/cas.13979, 2019 (PDF)

  • 2019/5/6(月)

    DgMab-6のエピトープマッピングの論文がアクセプトされました。

    Masato Sano, Mika K. Kaneko, Yukinari Kato*.
    (*corresponding author)

    Epitope Mapping of Anti-Human DGKgamma Monoclonal Antibody DgMab-6
    Monoclon. Antib. Immunodiagn. Immunother., in press  

    Abstract
    Diacylglycerol kinase (DGK) converts diacylglycerol (DG) into phosphatidic acid (PA). Both DG and PA serve as lipidic second messengers; therefore, DGK plays a critical role in regulating the balance of two signaling pathways mediated by DG and PA in cellular functions. DGKgamma, one of the 10 mammalian DGK isozymes, has been reported to be involved in membrane traffic, mast cell function, and leukemic cell differentiation. We previously developed a novel anti-DGKgamma monoclonal antibody (mAb), DgMab-6, which is very useful in immunocytochemistry of human cultured cells. In this study, we characterized the binding epitope of DgMab-6 using Western blotting, and Glu12 is important for facilitating the DgMab-6 binding to the DGKgamma protein. These results might lead to further development for sensitive and specific mAbs against DGKgamma.

  • 2019/5/5(日)

    自分の論文からの新しい引用
    High-affinity anti-glycan antibodies: challenges and strategies
    Z Polonskaya, PB Savage, MG Finn, L Teyton - Current Opinion in Immunology, 2019
    High-affinity binding of antibodies provides for increased specificity and usually higher effector functions in vivo. This goal, well documented in cancer immunotherapy, is very relevant to vaccines as well, and has particularly significant … Twitter Facebook

    Crystal structure of a mammalian Wnt–frizzled complex
    H Hirai, K Matoba, E Mihara, T Arimori, J Takagi - Nature Structural & Molecular …, 2019
    Wnt signaling plays fundamental roles in organogenesis, tissue regeneration and cancer, but high-resolution structural information of mammalian Wnt proteins is lacking. We solved a 2.8-Å resolution crystal structure of human Wnt3 in complex … Twitter Facebook

    [HTML] Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis
    Y Furusawa, J Takei, Y Sayama, S Yamada… - … and Biophysics Reports, 2019
    Sensitive and specific monoclonal antibodies (mAbs) targeting podoplanin (PDPN) are needed for immunohistochemical analyses using formalin-fixed paraffin- embedded tissues because PDPN is known as a lymphatic endothelial cell maker in …

  • 2019/5/5(日)

    抗ヤギポドプラニン抗体については、現在、2報が査読中です。
    (オニコウベにて撮影)

  • 2019/5/4(土)

    研究日記をお休みしている間に、複数の抗ポドプラニン抗体を作製しました。

    ・tiger PDPN: PMab-231 (establish)
    ・horse PDPN: PMab-219 (establish), PMab-202 (establish)
    ・alpca PDPN: PMab-225 (establish)
    ・pig PDPN: PMab-213 (establish), PMab-210 (establish)
    ・Tasmanian devil PDPN: PMab-233 (establish)
    ・whale PDPN: PMab-237 (establish)
    ・bear PDPN: PMab-247 (establish)

    *BINDSで開発中のCBIS法によって樹立しています。

  • 2019/5/4(土)

    PMab-237 (クジラポドプラニンに対する抗体)のIHC論文。
    Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Junko Takei, Masato Sano, Satoru Konnai, Atsushi Kobayashi, Shotaro Nakagun, Yoshiyasu Kobayashi, Mika K. Kaneko, Yukinari Kato*
    (*corresponding author)

    Immunohistochemical analysis of the harbor porpoise using anti-podoplanin antibody PMab-237
    Monoclon. Antib. Immunodiagn. Immunother., in press

    *今回の論文ではネズミイルカ(harbor porpoise)の切片を使用しています(帯広畜産大学から提供)。ちなみに、ネズミイルカはクジラの一種です。


    *以下の論文で、PMab-237を樹立しました。
    Yukinari Kato*, Yoshikazu Furusawa, Shunsuke Itai, Junko Takei, Takuro Nakamura, Masato Sano, Hiroyuki Harada, Shinji Yamada, Mika K. Kaneko
    (*corresponding author)

    Establishment of an anti-cetacean podoplanin monoclonal antibody PMab-237 for immunohistochemical analysis
    Monoclon. Antib. Immunodiagn. Immunother., in press

  • 2019/5/4(土)

    PMab-247 (クマラポドプラニンに対する抗体)の樹立論文。

    Yoshikazu Furusawa, Junko Takei, Yusuke Sayama, Shinji Yamada, Mika K. Kaneko, Yukinari Kato.*
    (*corresponding author)

    Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis
    Biochem Biophys Rep., Volume 18, July 2019, 100644, https://doi.org/10.1016/j.bbrep.2019.100644, 2019 (PDF)



    *クマ組織は北海道大学から提供を受けており、IHC論文は現在査読中です。

  • 2019/5/4(土)

    PMab-231 (トラポドプラニンに対する抗体)の樹立論文。

    Furusawa Y, Kaneko MK, Nakamura T, Itai S, Fukui M, Harada H, Yamada S, Kato Y.*
    (*corresponding author)

    Establishment of a monoclonal antibody PMab-231 for tiger podoplanin
    Monoclon. Antib. Immunodiagn. Immunother., 38(2):89–95, https://doi.org/10.1089/mab.2019.0003, 2019 (PDF)


    (シンガポール動物園にて撮影)

    *今回の論文では、ネコの組織(海外から購入)を使ってIHCの検討を行なっています。ネコとトラのポドプラニンの配列はほぼ同じです。

    *以下の論文において、ネコに対する抗ポドプラニン抗体PMab-52がトラ組織に交差反応性を示すことを報告しています。トラの組織については、山口大学から提供して頂いています。

    Yamada S, Itai S, Furusawa Y, Sano M, Nakamura T, Yanaka M, Handa S, Hisamatsu K, Nakamura Y, Fukui M, Harada H, Mizuno T, Sakai Y, Ogasawara S, Murata T, Uchida H, Tahara H, Kaneko MK, Kato Y.*
    (*corresponding author)

    Detection of tiger podoplanin using the anti-cat podoplanin monoclonal antibody PMab-52
    Monoclon. Antib. Immunodiagn. Immunother.,37(5):224–228, https://doi.org/10.1089/mab.2018.0033, 2018 (PDF)

  • 2019/5/4(土)

    PMab-225 (アルパカポドプラニンに対する抗体)の樹立論文。

    Kato Y*, Furusawa Y, Yamada S, Itai S, Takei J, Sano M, Kaneko MK.
    (*corresponding author)

    Establishment of a monoclonal antibody PMab-225 against alpaca podoplanin for immunohistochemical analyses
    Biochem Biophys Rep., Volume 18, July 2019, 100633, https://authors.elsevier.com/sd/article/S2405580818303194, 2019 (PDF)



    *アルパカの組織は、以下の仕事の際に北海道大学から提供を受けています。
    Kato Y*, Yamada S, Itai S, Konnai S, Kobayashi A, Kaneko MK.
    (*corresponding author)

    Detection of alpaca podoplanin by immunohistochemistry using the anti-bovine podoplanin monoclonal antibody PMab-44
    Monoclon. Antib. Immunodiagn. Immunother., 37(6); 269–271, doi: 10.1089/mab.2018.0037, 2018(PDF)

  • 2019/5/4(土)

    PMab-233 (タスマニアデビルポドプラニンに対する抗体)の樹立論文。

    Furusawa Y, Yamada S, Itai S, Nakamura T, Takei J, Sano M, Harada H, Fukui M, Kaneko MK, Kato Y. *
    (*corresponding author)

    Establishment of a monoclonal antibody PMab-233 for immunohistochemical analysis against Tasmanian devil podoplanin
    Biochem Biophys Rep., Volume 18, July 2019, 100631, https://doi.org/10.1016/j.bbrep.2019.100631 (PDF)



    *タスマニアデビル(絶滅危惧種)の組織は入手できませんでした。オーストラリアの研究者との共同研究を検討中です。

  • 2019/5/4(土)

    PMab-219 (ウマポドプラニンに対する抗体)のIHC論文。

    Kato Y*, Yamada S, Itai S, Kobayashi A, Konnai S, Kaneko MK.
    (*corresponding author)

    Anti-Horse Podoplanin Monoclonal Antibody PMab-219 is useful for detecting lymphatic endothelial cells by immunohistochemical analysis
    Monoclon. Antib. Immunodiagn. Immunother., 37(6); 272–274, https://doi.org/10.1089/mab.2018.0044, 2018 (PDF)

    *ウマの組織は、北海道大学から提供を受けています。

    (オニコウベにて撮影)

    *以下の論文でPMab-219を樹立しました。

    Furusawa Y, Yamada S, Itai S, Nakamura T, Yanaka M, Sano M, Harada H, Fukui M, Kaneko MK, Kato Y.*
    (*corresponding author)

    PMab-219: a monoclonal antibody for the immunohistochemical analysis of horse podoplanin
    Biochem Biophys Rep., 18:100616. doi: 10.1016/j.bbrep.2019.01.009., 2019 (PDF)

    *ウマの腎臓細胞は、以下の仕事の際に山口大学から提供を受けています。

    Furusawa Y, Yamada S, Itai S, Sano M, Nakamura T, Yanaka M, Handa S, Mizuno T, Maeda K, Fukui M, Harada H, Kaneko MK, Kato Y.*
    (*corresponding author)

    Establishment of Monoclonal Antibody PMab-202 against Horse Podoplanin
    Monoclon. Antib. Immunodiagn. Immunother.,37(5):233–237, https://doi.org/10.1089/mab.2018.0030, 2018 (PDF)

  • 2019/5/4(土)

    PMab-213 (ブタポドプラニンに対する抗体)の樹立論文。

    Kato Y*, Yamada S, Furusawa Y, Itai S, Nakamura T, Yanaka M, Sano M, Harada H, Fukui M, Kaneko MK.
    (*corresponding author)

    PMab-213: a monoclonal antibody for immunohistochemical analysis against pig podoplanin
    Monoclon. Antib. Immunodiagn. Immunother., 38(1), 18-24; https://doi.org/10.1089/mab.2018.0048, 2019 (PDF)



    *マイクロミニブタの組織は、以下の仕事の際に岐阜大学から提供を受けています。また、ブタの組織は、山口大学から提供を受けています。

    Furusawa Y, Yamada S, Itai S, Sano M, Nakamura T, Yanaka M, Fukui M, Harada H, Mizuno T, Sakai Y, Takasu M, Kaneko MK, Kato Y*.
    (*corresponding author)

    PMab-210: A Monoclonal Antibody Against Pig Podoplanin
    Monoclon. Antib. Immunodiagn. Immunother., 38(1), 30-36; https://doi.org/10.1089/mab.2018.0038, 2019 (PDF)

  • 2019/5/4(土)

  • 2019/5/1(水)

    令和になり、研究日記を再開します。(これまでの日記はブログ(2018-2019)に記載しています。)